Simultaneous localization of [3H]estradiol and neurophysin I or arginine vasopressin in hypothalamic neurons demonstrated by a combined technique of dry-mount autoradiography and immunohistochemistry.

A combined technique of dry-mount autoradiography and immunoperoxidase staining is described for the localization of steroid hormones and neuropeptides in the same brain section. With this procedure [3H]estradiol is localized in nuclei of neurophysin and arginine vasopressin producing cells of the mouse supraoptic and paraventricular nucleus. The results suggest a direct action of estradiol on neurohypophysial hormone producing cells. This immunoautoradiographic technique is suitable for the simultaneous localization of steroid hormones and neuropeptides in the brain.