Literature DB >> 6762207

The determination by radiochemical assay of argininosuccinase produced in an Escherichia coli system in vitro.

M J Zidwick, P Rogers.   

Abstract

A highly sensitive radiochemical assay used to measure the synthesis and regulation of the product of the argH gene, argininosuccinase, in an Escherichia coli system in vitro is described. With L-[guanidino-14C]argininosuccinic acid as a substrate, and in the presence of excess arginase and urease, 14CO2 is collected in a simply designed micro-vessel. With this method less than 1 nmol of product can be measured in the presence of various concentrations of L-arginine.

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Year:  1982        PMID： 6762207      PMCID： PMC1153893          DOI： 10.1042/bj2070529

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  16 in total

1.  Dual regulation by arginine of the expression of the Escherichia coli argECBH operon.

Authors:  R A Kryzek; P Rogers
Journal:  J Bacteriol       Date:  1976-04       Impact factor: 3.490

2.  Effect of arginine on the stability and size of argECBH messenger ribonucleic acid in Escherichia coli.

Authors:  R A Krzyzek; P Rogers
Journal:  J Bacteriol       Date:  1976-04       Impact factor: 3.490

3.  In vitro synthesis and repression of argininosuccinase in Escherichia coli K12; partial purification of the arginine repressor.

Authors:  N E Kelker; W K Maas; H L Yang; G Zubay
Journal:  Mol Gen Genet       Date:  1976-02-27

4.  The induction of arginase in Saccharomyces cerevisiae.

Authors:  P A Whitney; B Magasanik
Journal:  J Biol Chem       Date:  1973-09-10       Impact factor: 5.157

5.  Modified methods for the determination of carbamyl aspartate.

Authors:  L M Prescott; M E Jones
Journal:  Anal Biochem       Date:  1969-12       Impact factor: 3.365

6.  A radiometric assay for arginase.

Authors:  P Righetti; L De Luca; G Wolf
Journal:  Anal Biochem       Date:  1968-02       Impact factor: 3.365

7.  Rapid method for determination of arginase activity in tissue homogenates.

Authors:  J W Geyer; D Dabich
Journal:  Anal Biochem       Date:  1971-02       Impact factor: 3.365

8.  Separation and properties of argininosuccinate and its two anhydrides and their detection in biological materials.

Authors:  S Ratner; M Kunkemueller
Journal:  Biochemistry       Date:  1966-06       Impact factor: 3.162

9.  Regulation of arginase activity by intermediates of the arginine biosynthetic pathway in Neurospora crassa.

Authors:  J Mora; R Salceda; S Sanchez
Journal:  J Bacteriol       Date:  1972-06       Impact factor: 3.490

10.  Isolation of transducing particles of phi-80 bacteriophage that carry different regions of the Escherichia coli genome.

Authors:  R Press; N Glansdorff; P Miner; J De Vries; R Kadner; W K Maas
Journal:  Proc Natl Acad Sci U S A       Date:  1971-04       Impact factor: 11.205
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  2 in total

1.  Positive control of expression of the argECBH gene cluster in vitro by guanosine 5'-diphosphate 3'-diphosphate.

Authors:  M J Zidwick; J Korshus; P Rogers
Journal:  J Bacteriol       Date:  1984-08       Impact factor: 3.490

2.  Regulation and coupling of argECBH mRNA and enzyme synthesis in cell extracts of Escherichia coli.

Authors:  M J Zidwick; G Keller; P Rogers
Journal:  J Bacteriol       Date:  1984-08       Impact factor: 3.490
  2 in total

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