The ultrastructural meiotic phenotype of the radiation sensitive mutant rad 6-1 in yeast.

An ultrastructural analysis of three yeast rad 6-1/rad 6-1 diploids on sporulation medium for 0, 6, 10, and 24 h shows that arrest occurs at meiotic prophase. Two strains, CL 139 and PU 6, fail to complete chromosome synapsis based on the continued presence of single chromosomal cores in arrested nuclei. A clone derived from CL 139, however, showed complete pairing as evident from the presence of 17 synaptonemal complexes. All three strains underwent spindle pole body duplication but the poles failed to form a proper metaphase I spindle. A revertant Rad 6+ isolated from CL 139 showed normal chromosome behaviour and normal kinetic functions. It is concluded that the absence of meiotic recombination in some Rad 6- strains may result from asynapsis, but that in other strains (e.g., CL 139s) recombination fails in spite of complete synapsis. In all cases the lack of sporulation is adequately explained by failure of the kinetic apparatus to form a metaphase I spindle.