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Literature DB >> 6760128

A new RNA polymerase and in vitro transcription of the origin of replication from rat mitochondrial DNA.

K Yaginuma, M Kobayashi, M Taira, K Koike.

Abstract

A new RNA polymerase was found in a rat mitochondrial extract. This enzyme showed strong template preference in vitro for the supercoiled recombinant plasmid consisting of pBR322 and the D-loop region of rat mtDNA carrying the origin of heavy-strand replication. The main products synthesized by the D-loop region were two RNAs of different sizes. Both of these products were light-strand products transcribed from the region upstream from the origin of replication. This specific transcription is discussed in relation to initiation of primer RNA synthesis for heavy-strand replication of rat mtDNA.

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Year: 1982 PMID： 6760128 PMCID： PMC327028 DOI： 10.1093/nar/10.23.7531

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

18 in total

1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

2. Purification and properties of mitochondrial deoxyribonucleic acid dependent ribonucleic acid polymerase from ovaries of Xenopus laevis.

Authors: G J Wu; I B Dawid
Journal: Biochemistry Date: 1972-09-12 Impact factor: 3.162

3. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.

Authors: D B Clewell; D R Helinski
Journal: Proc Natl Acad Sci U S A Date: 1969-04 Impact factor: 11.205

4. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

Authors: P S Thomas
Journal: Proc Natl Acad Sci U S A Date: 1980-09 Impact factor: 11.205

5. Mechanism of mitochondrial DNA replication in mouse L-cells: localization and sequence of the light-strand origin of replication.

Authors: P A Martens; D A Clayton
Journal: J Mol Biol Date: 1979-12-05 Impact factor: 5.469

6. Mechanism of mitochondrial DNA replication in mouse L-cells: RNA priming during the initiation of heavy-strand synthesis.

Authors: A M Gillum; D A Clayton
Journal: J Mol Biol Date: 1979-12-05 Impact factor: 5.469

A new RNA polymerase and in vitro transcription of the origin of replication from rat mitochondrial DNA.

1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

2. Purification and properties of mitochondrial deoxyribonucleic acid dependent ribonucleic acid polymerase from ovaries of Xenopus laevis.

3. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.

4. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.

5. Mechanism of mitochondrial DNA replication in mouse L-cells: localization and sequence of the light-strand origin of replication.

6. Mechanism of mitochondrial DNA replication in mouse L-cells: RNA priming during the initiation of heavy-strand synthesis.

7. Nucleotide sequence of a region of human mitochondrial DNA containing the precisely identified origin of replication.

8. Cloning and characterization of the replication origin from rat mitochondrial DNA.

9. Mitochondrial DNA polymerase from rat liver.

10. Sequencing end-labeled DNA with base-specific chemical cleavages.

1. Decreased mitochondrial gene expression in isolated islets of rats injected neonatally with streptozotocin.

Review 2. Mitochondrial transcription initiation: promoter structures and RNA polymerases.