| Literature DB >> 6759370 |
S A Weiss, G C Smith, J L Vaughn, E M Dougherty, G J Tompkins.
Abstract
When IPL-SF-21AE III continuous insect cell line was grown and maintained in IPL-41 insect cell culture medium supplemented with 16 microM of AlCl3 or 0.24 microM of ZnSO4 . 7H2O, or both metallic salts, and then infected with Autographa california nuclear polyhedrosis virus, virus replication was increased significantly. The yield of polyhedral inclusion bodies (PIB) was enhanced up to 121%. Synthesis of cell-free nonoccluded virus was increased to 365% when infectivity was assayed by the plaque method. Newly applied electron microscopic quantitation and stereological techniques also revealed a significant increase in virus particles (VP) and in amount and size of PIB as well as number of VP per PIB.Entities:
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Year: 1982 PMID: 6759370 DOI: 10.1007/bf02796350
Source DB: PubMed Journal: In Vitro ISSN: 0073-5655