Simplified method for Chlamydia trachomatis isolation using multi-well plate.

To simplify Chlamydia trachomatis isolation, we employed a 24-well tissue culture plate, thereby eliminating tedious steps and material in the conventional vial method. Parallel studies indicated that the vial and plate methods were equally sensitive for Chlamydia isolation. The time for culturing McCoy cells was reduced from 72 to four hours allowing us to inoculate specimens the same day. The potential hazard of handling coverslips which might contain infected cells was avoided.