Fluorescence immunoassay in the clinical laboratory.

Currently applied to the measurement of proteins, hormones and drugs, fluorescence immunoassay (FIA) is a popular and fast-growing non-isotopic immunoassay technique. This paper describes a number of current FIA methodologies, including: homogeneous enzyme immunoassay with fluorescence detection; fluorogenic substrate detection: fluorescence polarization detection; fluorescent excitation transfer immunoassay; fluorescence protection immunoassay; and techniques that use solid-phase separation media.