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Literature DB >> 6752128

Bacteriophage P22 as a vector for Mu mutagenesis in Salmonella typhimurium: isolation of nad-lac and pnc-lac gene fusions.

Abstract

Salmonella phage P22 was utilized as a vector for phage Mu cts d1(Apr lac) mutagenesis in Salmonella typhimurium. Efficient transposition of phage Mu d1 and the construction of gene fusions were readily accomplished with this procedure. Mutants blocked in the biosynthesis of NAD+ and in pyridine nucleotide cycle metabolism were isolated by this method, resulting in nadB-lac, nadC-lac, and pncB-lac gene fusions.

Entities: Chemical Species

Mesh：

Substances：
NAD

Year: 1982 PMID： 6752128 PMCID： PMC221562 DOI： 10.1128/jb.152.2.959-962.1982

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

17 in total

1. Mapping and characterization of the nad genes in Salmonella typhimurium LT-2.

Authors: J W Foster; A G Moat
Journal: J Bacteriol Date: 1978-02 Impact factor: 3.490

Review 2. Linkage map of Salmonella typhimurium, edition V.

Authors: K E Sanderson; P E Hartman
Journal: Microbiol Rev Date: 1978-06

3. Biochemical genetics of the alpha-keto acid dehydrogenase complexes of Escherichia coli K12: isolation and biochemical properties of deletion mutants.

Authors: D Langley; J R Guest
Journal: J Gen Microbiol Date: 1977-04

4. Mapping of a gene causing resistance to chlorate in Salmonella typhimurium.

Authors: A H Stouthamer; C W Bettenhaussen
Journal: Antonie Van Leeuwenhoek Date: 1970 Impact factor: 2.271

5. A genetical and biochemical study of chlorate-resistant mutants of Salmonella typhimurium.

Authors: A H Stouthamer
Journal: Antonie Van Leeuwenhoek Date: 1969 Impact factor: 2.271

6. A method for detection of phage mutants with altered transducing ability.

Authors: H Schmieger
Journal: Mol Gen Genet Date: 1971

7. Transduction by phage P1CM clr-100 in Salmonella typhimurium.

Authors: T Mojica
Journal: Mol Gen Genet Date: 1975

8. Biochemical and genetic characterics of deletion and other mutant strains of Salmonella typhimurium LT2 lacking alpha-keto acid dehydrogenase complex activities,.

Authors: D Langley; J R Guest
Journal: J Gen Microbiol Date: 1974-06

9. Recognition of a gene involved in the regulation of nicotinamide adenine dinucleotide biosynthesis.

Authors: G J Tritz; J L Chandler
Journal: J Bacteriol Date: 1973-04 Impact factor: 3.490

10. De novo biosynthesis of nicotinamide adenine dinucleotide in Escherichia coli: excretion of quinolinic acid by mutants lacking quinolinate phosphoribosyl transferase.

Authors: J L Chandler; R K Gholson
Journal: J Bacteriol Date: 1972-07 Impact factor: 3.490

20 in total

1. Cyclic AMP receptor protein and TyrR are required for acid pH and anaerobic induction of hyaB and aniC in Salmonella typhimurium.

Authors: K R Park; J C Giard; J H Eom; S Bearson; J W Foster
Journal: J Bacteriol Date: 1999-01 Impact factor: 3.490

2. Acid shock induction of RpoS is mediated by the mouse virulence gene mviA of Salmonella typhimurium.

Authors: S M Bearson; W H Benjamin; W E Swords; J W Foster
Journal: J Bacteriol Date: 1996-05 Impact factor: 3.490

3. Unmasking of bacteriophage Mu lipopolysaccharide receptors in Salmonella enteritidis confers sensitivity to Mu and permits Mu mutagenesis.

Authors: K H Müller; T J Trust; W W Kay
Journal: J Bacteriol Date: 1988-03 Impact factor: 3.490

10. Mutagenesis of Erwinia carotovora subsp. carotovora with bacteriophage Mu d1 (Apr lac cts62): construction of his-lac gene fusions.

Authors: R K Jayaswal; R A Bressan; A K Handa
Journal: J Bacteriol Date: 1984-05 Impact factor: 3.490