Literature DB >> 6750131

Freeze-substitution.

D M Harvey.   

Abstract

Freeze-substitution is a technique suitable for the preparation of unicellular and multi-cellular plant and animal specimens for conventional light microscopy, TEM and SEM. It is also widely used as a means of preparing animal and plant tissues for the localization of water soluble substances by analytical electron microscopy, autoradiography or visual detection of precipitates. The technical requirements of preparation, together with an evaluation of the procedures, are presented for various applications. Careful selection and evaluation of freezing technique, substitution solvent and regime are required for meaningful results.

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Year:  1982        PMID： 6750131     DOI： 10.1111/j.1365-2818.1982.tb00414.x

Source DB:  PubMed          Journal:  J Microsc        ISSN： 0022-2720            Impact factor:   1.758


  26 in total

1.  The frog neuromuscular junction revisited after quick-freezing-freeze-drying: ultrastructure, immunogold labelling and high resolution calcium mapping.

Authors:  R Pezzati; F Grohovaz
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  1999-02-28       Impact factor: 6.237

2.  Differences in the width of the intercellular spaces in the epithelial basal infolding and the renal glomerular filtration site between freeze-substitution and conventional fixation.

Authors:  H Iwasa; H Kondo
Journal:  J Anat       Date:  1999-02       Impact factor: 2.610

Review 3.  Enzyme histochemistry and immunohistochemistry with freeze-dried or freeze-substituted resin-embedded tissue.

Authors:  G I Murray
Journal:  Histochem J       Date:  1992-07

4.  Quantitative evaluation of freeze-substitution effects on preservation of nuclear antigens during preparation of biological samples for immunoelectron microscopy.

Authors:  Margarita A Sobol; Vlada V Philimonenko; Anatoly A Philimonenko; Pavel Hozák
Journal:  Histochem Cell Biol       Date:  2012-03-01       Impact factor: 4.304

5.  Application of rapid freezing followed by freeze-substitution acrolein fixation for cytochemical studies of the rat stomach.

Authors:  F Murata; S Suzuki; S Tsuyama; T Suganuma; M Imada; C Furihata
Journal:  Histochem J       Date:  1985-09

6.  Structure of the cumulus oophorus at the time of fertilization.

Authors:  D M Phillips; V R Zacharopoulos; M E Perotti
Journal:  Cell Tissue Res       Date:  1990-08       Impact factor: 5.249

7.  Effect of chemical fixatives on accurate preservation of Escherichia coli and Bacillus subtilis structure in cells prepared by freeze-substitution.

Authors:  L L Graham; T J Beveridge
Journal:  J Bacteriol       Date:  1990-04       Impact factor: 3.490

8.  Response of Xylem Ray Parenchyma Cells of Red Osier Dogwood (Cornus sericea L.) to Freezing Stress (Microscopic Evidence of Protoplasm Contraction).

Authors:  Z. Ristic; E. N. Ashworth
Journal:  Plant Physiol       Date:  1994-02       Impact factor: 8.340

9.  Ultrastructural Evidence That Intracellular Ice Formation and Possibly Cavitation Are the Sources of Freezing Injury in Supercooling Wood Tissue of Cornus florida L.

Authors:  Z. Ristic; E. N. Ashworth
Journal:  Plant Physiol       Date:  1993-11       Impact factor: 8.340

10.  Immunohistochemical study of T lymphocyte subsets on frozen substituted plastic embedding section of the bone marrow from patients with myelodysplastic syndrome and its clinical implication.

Authors:  S Liu; X Liu; P Zou; S Song; B Wang
Journal:  J Tongji Med Univ       Date:  1999
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