The frequency of serological anti-zona pellucida activity in males, females and children.

945 human sera from children, fertile and infertile females, pregnant women and fertile and infertile males were tested for antibodies to porcine zona pellucida by immunofluorescence and the pronase-induced zona-lysis inhibition test. Positive sera were found in each of the groups examined, with a higher percentage of anti-zona activity in infertile, pregnant and menopausal women. There was no correlation between anti-sperm antibody titer and the incidence of anti-zona activity in either female or male human sera, nor did the occurrence of anti-zona activity correlate with a particular blood group type.