Literature DB >> 6749886

Antibody-mediated cellular cytotoxicity against Raji cells ADCC(Raji): evaluation of false positives in the detection of circulating immune complexes by Raji-cell assay.

M K Dasgupta, T Kovithavongs, J Schlaut, B M Longenecker, J B Dossetor.   

Abstract

Raji-cell radioimmunoassay is a very sensitive and reproducible method for the detection of circulating immune complexes. Using a complement-independent, antibody-dependent cellular cytotoxicity assay against 51Cr-labeled Raji cells, there is no correlation between activity against Raji cells and positivity in Raji-cell radioimmunoassay for circulating immune complexes in three sets of sera (from renal transplantation patients, multiparous women, and patients suffering from systemic lupus erythematosus). We conclude that IgG antibodies to Raji membranes are not a significant cause of false-positive results in circulating immune complexes as detected by Raji-cell radioimmunoassay.

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Year:  1982        PMID： 6749886     DOI： 10.1007/BF00915222

Source DB:  PubMed          Journal:  J Clin Immunol        ISSN： 0271-9142            Impact factor:   8.317


  22 in total

1.  Lymphocytotoxic antibodies in SLE patients and their relatives.

Authors:  I Malavé; R Papa; Z Layrisse
Journal:  Arthritis Rheum       Date:  1976 Jul-Aug

Review 2.  The biology and detection of immune complexes.

Authors:  A N Theofilopoulos; F J Dixon
Journal:  Adv Immunol       Date:  1979       Impact factor: 3.543

Review 3.  UCLA conference. Circulating immune complexes: their immunochemistry, detection, and importance.

Authors: 
Journal:  Ann Intern Med       Date:  1979-09       Impact factor: 25.391

4.  DTT test: a new method to differentiate IgM and IgG erythrocyte antibodies.

Authors:  B Pirofsky; E R Rosner
Journal:  Vox Sang       Date:  1974       Impact factor: 2.144

5.  Relationship of the clinical course of systemic lupus erythematosus to the presence of circulating lymphocytotoxic antibodies.

Authors:  W T Butler; J T Sharp; R D Rossen; M D Lidsky; K K Mittal; D A Gard
Journal:  Arthritis Rheum       Date:  1972 May-Jun

6.  Lymphocyte cytotoxic antibodies in systemic lupus erythematosus.

Authors:  K K Mittal; R D Rossen; J T Sharp; M D Lidsky; W T Butler
Journal:  Nature       Date:  1970-03-28       Impact factor: 49.962

7.  Lymphocytotoxic antibodies in systemic lupus erythematosus and clinically related diseases.

Authors:  A L Schocket; P F Kohler
Journal:  Arthritis Rheum       Date:  1979-10

8.  Detection of immune complexes in unheated sera by modified 125I-Clq binding test. Effect of heating on the binding of Clq by immune complexes and application of the test to systemic lupus erythematosus.

Authors:  R H Zubler; G Lange; P H Lambert; P A Miescher
Journal:  J Immunol       Date:  1976-01       Impact factor: 5.422

9.  Characterization of lymphocytotoxic antibodies in renal transplantation.

Authors:  R Roy; J G Lachance; Y Fradet; J Hebert
Journal:  Transplantation       Date:  1981-01       Impact factor: 4.939

10.  Radioconglutinin-binding azssay for circulating immune complexes: a new method.

Authors:  K V Johny; M K Dasgupta; B Singh; J B Dossetor
Journal:  Clin Exp Immunol       Date:  1980-06       Impact factor: 4.330
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  1 in total

1.  Combined evaluation of circulating immune complexes and antibodies to Pseudomonas aeruginosa as an immunologic profile in relation to pulmonary function in cystic fibrosis.

Authors:  M K Dasgupta; P Zuberbuhler; A Abbi; F L Harley; N E Brown; K Lam; J B Dossetor; J W Costerton
Journal:  J Clin Immunol       Date:  1987-01       Impact factor: 8.317
  1 in total

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