| Literature DB >> 6749339 |
E F Wakefield, M J Shelton, C S Hosking.
Abstract
The measurement of IgG, using a sandwich-type enzyme-linked immunosorbent assay (ELISA), is described. The assay is specific, sensitive, and simple to perform. Purification of the antibody, prior to attachment to the plastic solid phase, results in a more sensitive test. Optimum standardisation of the reagents and conditions is described and discussed. The assay is capable of detecting IgG to 2.5 X 10(-5) IU/ml (approximately equal to 3 ng/ml) and is useful for measuring IgG produced by cells in culture. The coefficient of variation within assays is 6.0% and between assays is 7.7%.Mesh：
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Year: 1982 PMID： 6749339 DOI： 10.1016/0009-8981(82)90175-9
Source DB: PubMed Journal: Clin Chim Acta ISSN： 0009-8981 Impact factor: 3.786