Literature DB >> 6746910

Molecular properties of the Ro/SSA antigen and enzyme-linked immunosorbent assay for quantitation of antibody.

H Yamagata, J B Harley, M Reichlin.   

Abstract

Antibodies to the Ro/SSA antigen occur in patients with systemic lupus erythematosus and Sjögren's syndrome. An immunoaffinity method for the preparation of electrophoretically homogeneous Ro/SSA antigen is described. Several molecular properties of the antigen have been determined. The native RNA protein particle has a molecular weight of approximately 100,000 D determined by gel filtration. Sodium dodecyl sulfate-analysis of the purified Ro/SSA antigen and analysis by staining of bands with silver and Coomassie Blue, Western blotting, and RNAase treatment leads to a hypothesis for the structure of the particle in which an antigenic 60,000 protein is bound to 24,000-27,000 RNA molecules which are not antigenic. An enzyme-linked immunoabsorbent assay method for assay of anti-Ro/SSA is also described which sensitively measures antigen binding at dilutions of sera containing anti-Ro/SSA precipitins up to 10(7) fold. Normal sera on average have 10(3) less binding activity.

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Year:  1984        PMID： 6746910      PMCID： PMC370515          DOI： 10.1172/JCI111460

Source DB:  PubMed          Journal:  J Clin Invest        ISSN： 0021-9738            Impact factor:   14.808


  33 in total

1.  Resolution of the identity of certain antigen-antibody systems in systemic lupus erythematosus and Sjögren's syndrome: an interlaboratory collaboration.

Authors:  M Alspaugh; P Maddison
Journal:  Arthritis Rheum       Date:  1979-07

2.  DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.

Authors:  B J DAVIS
Journal:  Ann N Y Acad Sci       Date:  1964-12-28       Impact factor: 5.691

3.  Metabolism of autologous and homologous IgG in rheumatoid arthritis.

Authors:  M A Catalano; E H Krick; D H De Heer; R M Nakamura; A N Theofilopoulos; J H Vaughan
Journal:  J Clin Invest       Date:  1977-08       Impact factor: 14.808

4.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

5.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

6.  Nucleotide-sequence analysis of Tn3 (ap): implications for insertion and deletion.

Authors:  H Ohtsubo; H Ohmori; E Ohtsubo
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1979

7.  Characterization of a soluble cytoplasmic antigen reactive with sera from patients with systemic lupus erythmatosus.

Authors:  G Clark; M Reichlin; T B Tomasi
Journal:  J Immunol       Date:  1969-01       Impact factor: 5.422

8.  Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G.

Authors:  E Engvall; P Perlmann
Journal:  Immunochemistry       Date:  1971-09

9.  Antibodies to cellular antigens in Sjögren's syndrome.

Authors:  M A Alspaugh; E M Tan
Journal:  J Clin Invest       Date:  1975-05       Impact factor: 14.808

10.  Anti-SS-A antibody and other antinuclear antibodies in systemic lupus erythematosus.

Authors:  E Scopelitis; J J Biundo; M A Alspaugh
Journal:  Arthritis Rheum       Date:  1980-03
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  48 in total

1.  Antibodies to extractable nuclear antigens. Has technological drift affected clinical interpretation?

Authors:  R J Lock; D J Unsworth
Journal:  J Clin Pathol       Date:  2001-03       Impact factor: 3.411

Review 2.  B-cell epitopes of La and Ro autoantigens.

Authors:  S Whittingham
Journal:  Mol Biol Rep       Date:  1992-06       Impact factor: 2.316

Review 3.  Autoantibodies against small cytoplasmic ribonucleoproteins: the anti-Ro/SS-A and anti-La/SS-B autoimmune response. A review of autoantibody detection, autoantigen composition, autoantibody-disease associations and possible etiologic mechanisms.

Authors:  J F Meilof
Journal:  Rheumatol Int       Date:  1992       Impact factor: 2.631

4.  Induction of anti-Ro60/anti-La by immunisation with spectrin and induction of anti-spectrin by immunisation with Ro60 and 4-hydroxy-2-nonenal-modified Ro60 immunisation.

Authors:  Biji T Kurien; Yaser Dorri; Michael Bachmann; R Hal Scofield
Journal:  Clin Exp Rheumatol       Date:  2012-12-17       Impact factor: 4.473

Review 5.  Significance of the Ro antigen system.

Authors:  M Reichlin
Journal:  J Clin Immunol       Date:  1986-09       Impact factor: 8.317

Review 6.  Autoantibodies to the RoRNP particles.

Authors:  M Reichlin
Journal:  Clin Exp Immunol       Date:  1995-01       Impact factor: 4.330

7.  Molecular characterization of human Ro/SS-A antigen. Amino terminal sequence of the protein moiety of human Ro/SS-A antigen and immunological activity of a corresponding synthetic peptide.

Authors:  T S Lieu; M M Newkirk; J D Capra; R D Sontheimer
Journal:  J Clin Invest       Date:  1988-07       Impact factor: 14.808

8.  Mapping of the human 60,000 M(r) Ro/SSA locus: the genes for three Ro/SSA autoantigens are located on separate chromosomes.

Authors:  M B Frank; M G Mattei
Journal:  Immunogenetics       Date:  1994       Impact factor: 2.846

9.  Autoantigenicity of Ro/SSA antigen is related to a nucleocapsid protein of vesicular stomatitis virus.

Authors:  R H Scofield; J B Harley
Journal:  Proc Natl Acad Sci U S A       Date:  1991-04-15       Impact factor: 11.205

10.  Anti-KJ: a new antibody associated with the syndrome of polymyositis and interstitial lung disease.

Authors:  I N Targoff; F C Arnett; L Berman; C O'Brien; M Reichlin
Journal:  J Clin Invest       Date:  1989-07       Impact factor: 14.808
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