Literature DB >> 6746574

Subcellular localization of alkaline phosphatase in Bacillus licheniformis 749/C by immunoelectron microscopy with colloidal gold.

G Tinglu, A Ghosh, B K Ghosh.   

Abstract

Subcellular distribution of the alkaline phosphatase of Bacillus licheniformis 749/C was determined by an immunoelectron microscopy method. Anti-alkaline phosphatase antibody labeled with 15- to 18-nm colloidal gold particles (gold-immunoglobulin G [IgG] complex) were used for the study. Both the plasma membrane and cytoplasmic material were labeled with the gold-IgG particles. These particles formed clusters in association with the plasma membrane; in contrast, in the cytoplasm the particles were largely dispersed, and only a few clusters were found. The gold-IgG binding was quantitatively estimated by stereological analysis of labeled, frozen thin sections. This estimation of a variety of control samples showed that the labeling was specific for the alkaline phosphatase. Cluster formation of the gold-IgG particles in association with the plasma membrane suggests that existence of specific alkaline phosphatase binding sites (receptors) in the plasma membrane of B. licheniformis 749/C.

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Year:  1984        PMID： 6746574      PMCID： PMC215696          DOI： 10.1128/jb.159.2.668-677.1984

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  22 in total

1.  Colloidal gold granules as markers for cell surface receptors in the scanning electron microscope.

Authors:  M Horisberger; J Rosset; H Bauer
Journal:  Experientia       Date:  1975-10-15

2.  Properties of the membrane-bound alkaline phosphatase from glucose- and lactate-grown cells of Bacillus subtilis SB 15.

Authors:  R Ghosh; A Ghosh; B K Ghosh
Journal:  J Biol Chem       Date:  1977-10-10       Impact factor: 5.157

Review 3.  Stereological principles for morphometry in electron microscopic cytology.

Authors:  E R Weibel
Journal:  Int Rev Cytol       Date:  1969

4.  Multiple marking of cell surface receptors by gold granules: simultaneous localization of three lectin receptors on human erythrocytes.

Authors:  M Horisberger; M Vonlanthen
Journal:  J Microsc       Date:  1979-01       Impact factor: 1.758

5.  Polymorphism of the mesosome in Bacillus licheniformis (749/C and 749). Influence of chemical fixation monitored by freeze-etching.

Authors:  B K Ghosh; N Nanninga
Journal:  J Ultrastruct Res       Date:  1976-07

6.  Coloidal gold, ferritin and peroxidase as markers for electron microscopic double labeling lectin techniques.

Authors:  J Roth; M Binder
Journal:  J Histochem Cytochem       Date:  1978-03       Impact factor: 2.479

7.  Changes in the membrane bound alkaline phosphatase of glucose and lactate grown vegetative cells of Bacillus subtilis SB15.

Authors:  A Ghosh; B K Ghosh
Journal:  Biochem Biophys Res Commun       Date:  1972-11-15       Impact factor: 3.575

8.  Improved procedures for immunoferritin labeling of ultrathin frozen sections.

Authors:  K T Tokuyasu; S J Singer
Journal:  J Cell Biol       Date:  1976-12       Impact factor: 10.539

9.  Frozen thin sections of fresh tissue for electron microscopy, with a description of pancreas and liver.

Authors:  A K Christensen
Journal:  J Cell Biol       Date:  1971-12       Impact factor: 10.539

10.  The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.

Authors:  E S REYNOLDS
Journal:  J Cell Biol       Date:  1963-04       Impact factor: 10.539
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  11 in total

1.  Subcellular localization of IpaC, an invasion plasmid antigen of Shigella dysenteriae type 1 and its in-vitro binding capability to mammalian cells.

Authors:  N M Shaikh; A N Ghosh; B Nandi; R Kumar
Journal:  World J Microbiol Biotechnol       Date:  1995-09       Impact factor: 3.312

2.  Immunocytochemical ultrastructural analysis of chromatophore membrane formation in Rhodospirillum rubrum.

Authors:  S M Crook; S B Treml; M L Collins
Journal:  J Bacteriol       Date:  1986-07       Impact factor: 3.490

3.  Ultrastructural localization of dipicolinic acid in dormant spores of Bacillus subtilis by immunoelectron microscopy with colloidal gold particles.

Authors:  S Kozuka; Y Yasuda; K Tochikubo
Journal:  J Bacteriol       Date:  1985-06       Impact factor: 3.490

4.  Immunoelectron microscopic double labeling of alkaline phosphatase and penicillinase with colloidal gold in frozen thin sections of Bacillus licheniformis 749/C.

Authors:  T Guan; A Ghosh; B K Ghosh
Journal:  J Bacteriol       Date:  1985-10       Impact factor: 3.490

5.  Localization of acyl carrier protein in Escherichia coli.

Authors:  S Jackowski; H H Edwards; D Davis; C O Rock
Journal:  J Bacteriol       Date:  1985-04       Impact factor: 3.490

6.  Labelling of colloidal gold with protein A. A quantitative study.

Authors:  M Horisberger; M F Clerc
Journal:  Histochemistry       Date:  1985

7.  Phosphate starvation enhances expression of the immunodominant 38-kilodalton protein antigen of Mycobacterium tuberculosis: demonstration by immunogold electron microscopy.

Authors:  C Espitia; M Elinos; R Hernández-Pando; R Mancilla
Journal:  Infect Immun       Date:  1992-07       Impact factor: 3.441

8.  Negative staining and immunoelectron microscopy of adhesion-deficient mutants of Streptococcus salivarius reveal that the adhesive protein antigens are separate classes of cell surface fibril.

Authors:  A H Weerkamp; P S Handley; A Baars; J W Slot
Journal:  J Bacteriol       Date:  1986-03       Impact factor: 3.490

9.  Structural properties of fibrillar proteins isolated from the cell surface and cytoplasm of Streptococcus salivarius (K+) cells and nonadhesive mutants.

Authors:  A H Weerkamp; H C van der Mei; R S Liem
Journal:  J Bacteriol       Date:  1986-03       Impact factor: 3.490

10.  Localization to the inner surface of the cytoplasmic membrane by immunoelectron microscopy of enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system of Escherichia coli.

Authors:  B K Ghosh; K Owens; R Pietri; A Peterkofsky
Journal:  Proc Natl Acad Sci U S A       Date:  1989-02       Impact factor: 11.205
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