Literature DB >> 673861

Measurement of the differential melting profile of a promoter-containing fragment of T7 DNA by means of a microspectrophotometer.

M A Grachev, M P Perelroyzen.   

Abstract

A double-beam microspectrophotometer with a 5 microliter cell has been used to study denaturation of DNA in an aqueous solution. This instrument enables measurement of high-resolution differential melting profiles simultaneously at several wavelengths with 0.5 to 1 microgram of DNA. Therefore it becomes possible to study nucleic acids which are difficult to obtain in large amounts. The techniques have been employed to measure the differential melting profiles of T7 DNA and of a fragment of this DNA 1000 base pairs long which contains the four early promoters.

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Year:  1978        PMID： 673861      PMCID： PMC342185          DOI： 10.1093/nar/5.7.2557

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  9 in total

1.  High resolution thermal denaturation of DNA: thermalites of bacteriophage DNA.

Authors:  D L Vizard; A T Ansevin
Journal:  Biochemistry       Date:  1976-02-24       Impact factor: 3.162

2.  Fine structures in denaturation curves of bacteriophage lambda DNA. Their relation to the intramolecular heterogeneity in base compositon.

Authors:  S Yabuki; O Gotoh; A Wada
Journal:  Biochim Biophys Acta       Date:  1975-07-07

3.  [Transcription of early genes of phage T7 DNA].

Authors:  A A Aleksandrov; D I Chernyi
Journal:  Dokl Akad Nauk SSSR       Date:  1976

4.  An expanded transcriptional map of T7 bacteriophage. Reading of minor T7 promoter sites in vitro by Escherichia coli RNA polymerase.

Authors:  S J Stahl; M J Chamberlin
Journal:  J Mol Biol       Date:  1977-06-05       Impact factor: 5.469

5.  Melting fine structure of DNA fragments of known base sequence from theta X174.

Authors:  A Wada; H Tachibana; S Ueno; Y Husimi; Y Machida
Journal:  Nature       Date:  1977-09-22       Impact factor: 49.962

Review 6.  [Molecular melting of DNA and the effect of the fine structure of fusion curves].

Authors:  Iu S Lazurkin
Journal:  Mol Biol (Mosk)       Date:  1977 Nov-Dec

7.  Spectral analysis on the melting fine structure of is lambda DNA and T2 DNA.

Authors:  C Akiyama; O Gotoh; A Wada
Journal:  Biopolymers       Date:  1977-02       Impact factor: 2.505

8.  [Isolation of promotor and terminator fragments of T7 phage DNA from a hydrolysate obtained by exposure to restriction endonuclease BsuR].

Authors:  M A Gravchev; E F Zaĭchikov; V V Kravchenko; A G Pletnev
Journal:  Dokl Akad Nauk SSSR       Date:  1978

9.  Specific fragmentation of T7 phage DNA at low-melting sites.

Authors:  V M Pavlov; Y L Lyubchenko; A S Borovik; Y S Lazurkin
Journal:  Nucleic Acids Res       Date:  1977-11       Impact factor: 16.971
  9 in total
  2 in total

1.  Localization of low-melting regions in phage T7 DNA.

Authors:  V I Lyamichev; I G Panyutin; D I Cherny
Journal:  Nucleic Acids Res       Date:  1983-04-11       Impact factor: 16.971

2.  A study of the reversibility of helix-coil transition in DNA.

Authors:  M P Perelroyzen; V I Lyamichev; A V Vologodskii
Journal:  Nucleic Acids Res       Date:  1981-08-25       Impact factor: 16.971
  2 in total

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