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Literature DB >> 6736225

Serological identification of oral Bacteroides spp. by enzyme-linked immunosorbent assay.

J L Ebersole, D E Frey, M A Taubman, D J Smith, S S Socransky, A C Tanner.

Abstract

A rapid method for identifying black-pigmented oral Bacteroides spp. is described. Species-specific rabbit antisera to Bacteroides gingivalis, B. intermedius, and B. melaninogenicus were used in an enzyme-linked immunosorbent assay to identify clinical isolates of black-pigmented Bacteroides spp. from humans. The results showed excellent agreement with biochemical identification of B. gingivalis and B. intermedius. Only 36% of the B. melaninogenicus isolates were identified with the enzyme-linked immunosorbent assay, suggesting that this group of black-pigmented Bacteroides spp. is made up of more than one serotype. The serological enzyme-linked immunosorbent assay should enable rapid identification of black-pigmented Bacteroides spp. isolated from sites of oral diseases and may also be used to identify the presence of these organisms in complex bacterial mixtures from oral sites.

Entities: Disease Species

Mesh：

Substances：

Year: 1984 PMID： 6736225 PMCID： PMC271146 DOI： 10.1128/jcm.19.5.639-644.1984

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

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