Literature DB >> 6734665

Radiochemistry and biostability of autologous leucocytes labelled with 99mTc-stannous colloid in whole blood.

R Hanna, T Braun, A Levendel, F Lomas.   

Abstract

Autologous leucocytes were labelled in whole blood by phagocytic uptake of 99mTc-stannous colloid. This colloid has a mean particle size of 1.5 micron and labels leucocytes with 81% efficiency. Individual cell uptakes were: granulocytes 42%, monocytes 39%. Isotonic sodium citrate added after the labelling procedure did solubilise excess colloid but was not necessary for adequate removal of excess colloid. Labelled leucocytes were shown in vitro to be viable and maintain normal bactericidal and chemotactic capacity. Biodistribution, clearance rates and dosimetry are presented. These results indicate that autologous leucocytes can be efficiently labelled with 99mTc-stannous colloid with good residual cell function.

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Year:  1984        PMID： 6734665     DOI： 10.1007/BF00448542

Source DB:  PubMed          Journal:  Eur J Nucl Med        ISSN： 0340-6997


  9 in total

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Authors:  R D Nelson; P G Quie; R L Simmons
Journal:  J Immunol       Date:  1975-12       Impact factor: 5.422

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Authors:  A Boyum
Journal:  Tissue Antigens       Date:  1974

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Authors:  D English; B R Andersen
Journal:  J Nucl Med       Date:  1977-03       Impact factor: 10.057

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Authors:  H J Schroth; E Oberhausen; R Berberich
Journal:  Eur J Nucl Med       Date:  1981

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Authors:  J G McAfee; M L Thakur
Journal:  J Nucl Med       Date:  1976-06       Impact factor: 10.057

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Authors:  B R Andersen; D English; H E Akalin; W Henderson
Journal:  Arch Intern Med       Date:  1975-08

7.  Indium-111-labeled human polymorphonuclear leukocytes: viability, random migration, chemotaxis, bacterial capacity, and ultrastructure.

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Journal:  J Nucl Med       Date:  1979-07       Impact factor: 10.057

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Authors:  M L Thakur; R E Coleman; M J Welch
Journal:  J Lab Clin Med       Date:  1977-01

9.  Effect of high doses of radiation on human neutrophil chemotaxis, phagocytosis and morphology.

Authors:  T R Holley; D E Van Epps; R L Harvey; R E Anderson; R C Williams
Journal:  Am J Pathol       Date:  1974-04       Impact factor: 4.307
  9 in total
  9 in total

Review 1.  Role of scintigraphy in inflammatory bowel disease.

Authors:  Maria I Stathaki; Sophia I Koukouraki; Nikolaos S Karkavitsas; Ioannis E Koutroubakis
Journal:  World J Gastroenterol       Date:  2009-06-14       Impact factor: 5.742

2.  Technetium-99m autologous phagocyte scanning: a new imaging technique for inflammatory bowel disease.

Authors:  W Pullman; R Hanna; P Sullivan; J A Booth; F Lomas; W F Doe
Journal:  Br Med J (Clin Res Ed)       Date:  1986-07-19

3.  Technetium-99m autologous phagocyte scanning: a new imaging technique for inflammatory bowel disease.

Authors:  A M Peters; J P Lavender; H J Danpure; S Osman; S H Saverymuttu
Journal:  Br Med J (Clin Res Ed)       Date:  1986-08-16

4.  Labelling of leucocytes with colloidal technetium-99m-SnF2: an investigation of the labelling process by autoradiography.

Authors:  M R Puncher; P J Blower
Journal:  Eur J Nucl Med       Date:  1995-02

Review 5.  Radionuclide targeting and dosimetry at the microscopic level: the role of microautoradiography.

Authors:  M R Puncher; P J Blower
Journal:  Eur J Nucl Med       Date:  1994-12

6.  Identification of factors affecting technetium 99m leucocyte labelling by phagocytic engulfment and development of an optimal technique.

Authors:  R W Hanna; F E Lomas
Journal:  Eur J Nucl Med       Date:  1986

7.  Evaluation of white cell scintigraphy using indium-111 and technetium-99m labelled leucocytes.

Authors:  S J Boyd; R Nour; R J Quinn; E McKay; S P Butler
Journal:  Eur J Nucl Med       Date:  1993-03

8.  Value of positive technetium-99m leucocyte scans in predicting intestinal inflammation.

Authors:  P Gibson; M Lichtenstein; N Salehi; G Hebbard; J Andrews
Journal:  Gut       Date:  1991-12       Impact factor: 23.059

9.  Examination of trafficking of phagocytosed colloid particles in neutrophils using synchrotron-based X-ray fluorescence microscopy (XFM).

Authors:  Stuart C Ramsay; Nathan Cassidy; Martin D de Jonge; Daryl L Howard; David Paterson; Natkunam Ketheesan
Journal:  J Biol Phys       Date:  2011-08-26       Impact factor: 1.365
  9 in total

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