Literature DB >> 6733267

A cell line secreting stimulating factors for CFU-GEMM culture.

C D Myers, F E Katz, G Joshi, J L Millar.   

Abstract

The multipotent hemopoietic stem cell has fastidious growth requirements in vitro. Traditionally, phytohemagglutinin-stimulated leukocyte conditioned medium has been used to supply the undefined growth factors required for culture of the human multipotent hemopoietic progenitor. We describe the use of medium conditioned by the bladder carcinoma cell line, 5637, to replace PHA-LCM in CFU-GEMM cultures and show that the properties of this conditioned medium closely mimic those of PHA-LCM in two separate CFU-GEMM culture systems.

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Year:  1984        PMID： 6733267

Source DB:  PubMed          Journal:  Blood        ISSN： 0006-4971            Impact factor:   22.113


  21 in total

1.  Simple method for granulocyte-macrophage cell culture and staining in soft agar: comparison with a standard research technique.

Authors:  K Hyde; A J Steed; H Lenehan; M J Saunders; J T Richards; J A Lin Yin; C G Geary
Journal:  J Clin Pathol       Date:  1989-12       Impact factor: 3.411

2.  Purification of hemopoietin 1: a multilineage hemopoietic growth factor.

Authors:  P T Jubinsky; E R Stanley
Journal:  Proc Natl Acad Sci U S A       Date:  1985-05       Impact factor: 11.205

3.  The role of CFU-GEMM in human hemopoiesis.

Authors:  H A Messner
Journal:  Blut       Date:  1986-10

Review 4.  Human granulocyte colony stimulating factor.

Authors:  E Platzer; J R Kalden
Journal:  Blut       Date:  1987-03

5.  Human recombinant granulocyte-macrophage colony stimulating factor and interleukin 3 have overlapping but distinct hematopoietic activities.

Authors:  S G Emerson; Y C Yang; S C Clark; M W Long
Journal:  J Clin Invest       Date:  1988-10       Impact factor: 14.808

6.  Restoration of normal lysosomal function in mucopolysaccharidosis type VII cells by retroviral vector-mediated gene transfer.

Authors:  J H Wolfe; E H Schuchman; L E Stramm; E A Concaugh; M E Haskins; G D Aguirre; D F Patterson; R J Desnick; E Gilboa
Journal:  Proc Natl Acad Sci U S A       Date:  1990-04       Impact factor: 11.205

7.  An assessment of myeloid colony-forming-cell generation in liquid human bone marrow cultures: influence of accessory cells and cytokines IL-1 alpha, beta and IL-6.

Authors:  R P Gooding; P G Riches; M Y Gordon
Journal:  Int J Exp Pathol       Date:  1993-04       Impact factor: 1.925

8.  An assessment of the ability of human bone marrow cultures to generate osteoclasts.

Authors:  A M Flanagan; M A Horton; E L Dorey; D A Collins; R S Evely; J M Moseley; F C Firkin; T J Chambers; M H Helfrich; T J Martin
Journal:  Int J Exp Pathol       Date:  1992-06       Impact factor: 1.925

9.  The toxicity of amodiaquine and its principal metabolites towards mononuclear leucocytes and granulocyte/monocyte colony forming units.

Authors:  P A Winstanley; J W Coleman; J L Maggs; A M Breckenridge; B K Park
Journal:  Br J Clin Pharmacol       Date:  1990-04       Impact factor: 4.335

10.  Karyotype and ultrastructure of a colony stimulating factor (CSF) producing cell line (5637) originated from a carcinoma of the human urinary bladder.

Authors:  K H Pflüger; H D Probeck; G Adler; D Stach-Machado; H Kapmeyer; K Havemann
Journal:  Blut       Date:  1986-08
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