Inhibition of gastric acid secretion by a phorbol ester: effect of 12-O-tetradecanoylphorbol-13-acetate on aminopyrine accumulation by rat parietal cells.

12-O-tetradecanoylphorbol-13-acetate ( 32nM ) inhibits aminopyrine accumulation induced by dibutyryl cyclic AMP or by histamine plus 3-isobutyl-1-methylxanthine in isolated rat parietal cells, but a significant effect on carbachol-stimulated aminopyrine accumulation is only seen at higher concentrations of the phorbol ester. 12-O-tetradecanoylphorbol-13-acetate ( 32nM ) does not affect aminopyrine accumulation or glucose oxidation in unstimulated cells, nor does it alter the ability of cells to exclude trypan blue. The phorbol ester may trigger a specific, and novel, parietal cell system capable of modulating the acid secretory response to histamine.