Literature DB >> 6731809

Determination of catechol-O-methyltransferase activity by high-performance liquid chromatography with electrochemical detection.

E Nissinen, P Männistö.   

Abstract

A rapid and simple assay for catechol-O-methyltransferase (COMT) activity by high-performance liquid chromatography with electrochemical detection is described. The method is based on the measurement of 3-O- and 4-O-methylated products (3-methoxy-4-hydroxy- and 4-methoxy-3-hydroxybenzylamine, respectively) of the substrate 3,4-dihydroxybenzylamine. These compounds are determined from the incubation mixture after removal of protein injecting aliquot into the liquid chromatograph. The detection limit is 1 pmol of product. This method is very suitable for screening of COMT activity as well as for determining the meta/para product ratios.

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Year:  1984        PMID： 6731809     DOI： 10.1016/0003-2697(84)90348-8

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  7 in total

1.  Inhibitors of catechol-O-methyltransferase sensitize mice to pain.

Authors:  O Kambur; R Talka; O B Ansah; V K Kontinen; A Pertovaara; E Kalso; P T Männistö
Journal:  Br J Pharmacol       Date:  2010-12       Impact factor: 8.739

2.  Striatal membrane-bound and soluble catechol-O-methyl-transferase after selective neuronal lesions in the rat.

Authors:  S Kaakkola; P T Männistö; E Nissinen
Journal:  J Neural Transm       Date:  1987       Impact factor: 3.575

3.  Importance of membrane-bound catechol-O-methyltransferase in L-DOPA metabolism: a pharmacokinetic study in two types of Comt gene modified mice.

Authors:  M Käenmäki; A Tammimäki; J A Garcia-Horsman; T Myöhänen; N Schendzielorz; M Karayiorgou; J A Gogos; P T Männistö
Journal:  Br J Pharmacol       Date:  2009-12       Impact factor: 8.739

4.  Inhibition of human erythrocyte and gastroduodenal catechol-O-methyltransferase activity by nitecapone.

Authors:  E Schultz; S Tarpila; A C Bäckström; A Gordin; E Nissinen; P Pohto
Journal:  Eur J Clin Pharmacol       Date:  1991       Impact factor: 2.953

5.  Over-expression of a human chromosome 22q11.2 segment including TXNRD2, COMT and ARVCF developmentally affects incentive learning and working memory in mice.

Authors:  Go Suzuki; Kathryn M Harper; Takeshi Hiramoto; Birgit Funke; MoonSook Lee; Gina Kang; Mahalah Buell; Mark A Geyer; Raju Kucherlapati; Bernice Morrow; Pekka T Männistö; Soh Agatsuma; Noboru Hiroi
Journal:  Hum Mol Genet       Date:  2009-07-19       Impact factor: 6.150

Review 6.  Analytical methodologies for sensing catechol-O-methyltransferase activity and their applications.

Authors:  Fang-Yuan Wang; Ping Wang; Dong-Fang Zhao; Frank J Gonzalez; Yu-Fan Fan; Yang-Liu Xia; Guang-Bo Ge; Ling Yang
Journal:  J Pharm Anal       Date:  2020-04-07

7.  Reduced natriuretic response to acute sodium loading in COMT gene deleted mice.

Authors:  Cecilia Odlind; Ilkka Reenilä; Pekka T Männistö; Risto Juvonen; Staffan Uhlén; Joseph A Gogos; Maria Karayiorgou; Peter Hansell
Journal:  BMC Physiol       Date:  2002-08-21
  7 in total

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