Literature DB >> 6731805

Purification and analysis of cobamides of Methanobacterium bryantii by high-performance liquid chromatography.

W B Whitman, R S Wolfe.   

Abstract

Cobamides from Methanobacterium bryantii were purified by isocratic reverse-phase high-performance liquid chromatography at neutral pH in readily extractable buffers. A gradient adaptation of this chromatography system on C-18 muBondapak columns in buffers containing aqueous methanol and 100 mM LiCl readily separated Co alpha-(5-hydroxybenzimidazoyl)-Co beta-cyanocobamide, Co alpha-(5-hydroxybenzimidazoyl)-Co beta-adenosylcobamide, cyanocobalamin, adenosylcobalamin, and methylcobalamin. Aquacobalamin was not resolved from cyanocobalamin by this procedure. The gradient procedure was also useful in following the fate of tritiated cyanocobalamin in cell-free extracts of M. bryantii.

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Year:  1984        PMID： 6731805     DOI： 10.1016/0003-2697(84)90380-4

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  7 in total

Review 1.  Methanogens and the diversity of archaebacteria.

Authors:  W J Jones; D P Nagle; W B Whitman
Journal:  Microbiol Rev       Date:  1987-03

2.  Identification of specific corrinoids reveals corrinoid modification in dechlorinating microbial communities.

Authors:  Yujie Men; Erica C Seth; Shan Yi; Terence S Crofts; Robert H Allen; Michiko E Taga; Lisa Alvarez-Cohen
Journal:  Environ Microbiol       Date:  2014-06-02       Impact factor: 5.491

3.  Refined experimental annotation reveals conserved corrinoid autotrophy in chloroform-respiring Dehalobacter isolates.

Authors:  Po-Hsiang Wang; Shuiquan Tang; Kayla Nemr; Robert Flick; Jun Yan; Radhakrishnan Mahadevan; Alexander F Yakunin; Frank E Löffler; Elizabeth A Edwards
Journal:  ISME J       Date:  2016-11-29       Impact factor: 10.302

4.  Substitution of Co alpha-(5-hydroxybenzimidazolyl)cobamide (factor III) by vitamin B12 in Methanobacterium thermoautotrophicum.

Authors:  E Stupperich; I Steiner; H J Eisinger
Journal:  J Bacteriol       Date:  1987-07       Impact factor: 3.490

5.  Acetate-dependent methylation of two corrinoid proteins in extracts of Methanosarcina barkeri.

Authors:  X J Cao; J A Krzycki
Journal:  J Bacteriol       Date:  1991-09       Impact factor: 3.490

6.  Purification, characterization, and molecular cloning of S-adenosyl-L-methionine: uroporphyrinogen III methyltransferase from Methanobacterium ivanovii.

Authors:  F Blanche; C Robin; M Couder; D Faucher; L Cauchois; B Cameron; J Crouzet
Journal:  J Bacteriol       Date:  1991-08       Impact factor: 3.490

7.  Activation of the methylreductase system from Methanobacterium bryantii by corrins.

Authors:  W B Whitman; R S Wolfe
Journal:  J Bacteriol       Date:  1985-10       Impact factor: 3.490
  7 in total

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