Literature DB >> 6726810

Visualization and exact molecular weight determination of a Rhizobium meliloti megaplasmid.

B Burkardt, H J Burkardt.   

Abstract

The entire DNA of Rhizobium meliloti MVII /1 cells was isolated preparatively by gentle lysis and sucrose gradient centrifugation. Fractions of the sucrose gradient were investigated by electron microscopy. We found intact megaplasmids in supercoiled and relaxed form and total chromosomes. The length of the megaplasmid could be measured, which allowed an exact determination of the molecular weight. We found a length of 0.48 +/- 0.019 mm equivalent to a molecular weight of about 1000 X 10(6).

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Year:  1984        PMID： 6726810     DOI： 10.1016/0022-2836(84)90475-3

Source DB:  PubMed          Journal:  J Mol Biol        ISSN： 0022-2836            Impact factor:   5.469


  19 in total

1.  Megaplasmid pRme2011a of Sinorhizobium meliloti is not required for viability.

Authors:  I J Oresnik; S L Liu; C K Yost; M F Hynes
Journal:  J Bacteriol       Date:  2000-06       Impact factor: 3.490

2.  Rhizobium meliloti genes involved in sulfate activation: the two copies of nodPQ and a new locus, saa.

Authors:  J S Schwedock; S R Long
Journal:  Genetics       Date:  1992-12       Impact factor: 4.562

3.  Analysis of Rhizobium meliloti Sym Mutants Obtained by Heat Treatment.

Authors:  N Toro; J Olivares
Journal:  Appl Environ Microbiol       Date:  1986-05       Impact factor: 4.792

4.  The composition of fluorescent pseudomonad populations associated with roots is influenced by plant and soil type.

Authors:  X Latour; T Corberand; G Laguerre; F Allard; P Lemanceau
Journal:  Appl Environ Microbiol       Date:  1996-07       Impact factor: 4.792

5.  Transcription patterns of Rhizobium meliloti symbiotic plasmid pSym: identification of nifA-independent fix genes.

Authors:  M David; O Domergue; P Pognonec; D Kahn
Journal:  J Bacteriol       Date:  1987-05       Impact factor: 3.490

6.  Diversity of Plasmid Profiles and Conservation of Symbiotic Nitrogen Fixation Genes in Newly Isolated Rhizobium Strains Nodulating Sulla (Hedysarum coronarium L.).

Authors:  T Mozo; E Cabrera; T Ruiz-Argüeso
Journal:  Appl Environ Microbiol       Date:  1988-05       Impact factor: 4.792

7.  Physical and genetic mapping of the Rhodobacter sphaeroides 2.4.1 genome: genome size, fragment identification, and gene localization.

Authors:  A Suwanto; S Kaplan
Journal:  J Bacteriol       Date:  1989-11       Impact factor: 3.490

8.  A new symbiotic cluster on the pSym megaplasmid of Rhizobium meliloti 2011 carries a functional fix gene repeat and a nod locus.

Authors:  M H Renalier; J Batut; J Ghai; B Terzaghi; M Gherardi; M David; A M Garnerone; J Vasse; G Truchet; T Huguet
Journal:  J Bacteriol       Date:  1987-05       Impact factor: 3.490

9.  The genomes of the family Rhizobiaceae: size, stability, and rarely cutting restriction endonucleases.

Authors:  B W Sobral; R J Honeycutt; A G Atherly
Journal:  J Bacteriol       Date:  1991-01       Impact factor: 3.490

10.  Physical map of the genome of Rhizobium meliloti 1021.

Authors:  R J Honeycutt; M McClelland; B W Sobral
Journal:  J Bacteriol       Date:  1993-11       Impact factor: 3.490
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