Literature DB >> 6725603

Evaluation of a commercial antibody capture enzyme immunoassay for the detection of rubella specific IgM.

J Hodgson, P Morgan-Capner.   

Abstract

A commercial M antibody capture ELISA kit ( Rubenz M) for the detection of rubella specific IgM was evaluated in comparison with M antibody capture radioimmunoassay. A total of 248 sera were evaluated, including sera from cases of primary postnatal rubella, congenital rubella, infectious mononucleosis, and sera which contained rheumatoid factor. No false positive results were obtained but two high positive sera gave Rubenz M values which were below the value recommended as indicative of a positive result. We therefore propose changes in the criteria used for assessing the significance of the results obtained. These changes improve the accuracy of the assay without loss of specificity.

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Year:  1984        PMID： 6725603      PMCID： PMC498785          DOI： 10.1136/jcp.37.5.573

Source DB:  PubMed          Journal:  J Clin Pathol        ISSN： 0021-9746            Impact factor:   3.411


  20 in total

1.  Determination of virus-specific IgM antibodies by using ELISA: elimination of false-positive results with protein A-Sepharose absorption and subsequent IgM antibody assay.

Authors:  P O Leinikki; I Shekarchi; P Dorsett; J L Sever
Journal:  J Lab Clin Med       Date:  1978-12

2.  Serum IgM and IgG responses in postnatally acquired rubella.

Authors:  J M Best; J E Banatvala; D Watson
Journal:  Lancet       Date:  1969-07-12       Impact factor: 79.321

3.  Comparison of immunofluorescence and radioimmunoassay for detecting IgM antibody in infants with the congenital rubella syndrome.

Authors:  J E Cradock-Watson; M K Ridehalgh; J R Pattison; M J Anderson; H O Kangro
Journal:  J Hyg (Lond)       Date:  1979-12

4.  The detection of rubella-specific IgM antibodies by radioimmunoassay.

Authors:  H O Kangro; J R Pattison; R B Heath
Journal:  Br J Exp Pathol       Date:  1978-12

5.  Rubella-specific IgM detection using Sephacryl S-300 gel filtration.

Authors:  P Morgan-Capner; E Davies; J R Pattison
Journal:  J Clin Pathol       Date:  1980-11       Impact factor: 3.411

6.  Rubella antibody measured by radial haemolysis. Characteristics and performance of a simple screening method for use in diagnostic laboratories.

Authors:  J B Kurtz; P P Mortimer; P R Mortimer; P Morgan-Capner; M S Shafi; G B White
Journal:  J Hyg (Lond)       Date:  1980-04

7.  Rubella-specific IgM reactivity in sera from cases of infectious mononucleosis.

Authors:  P Morgan-Capner; R S Tedder; J E Mace
Journal:  J Hyg (Lond)       Date:  1983-06

8.  A solid-phase immunosorbent technique for the rapid detection of rubella IgM by haemagglutination inhibition.

Authors:  U Krech; J A Wilhelm
Journal:  J Gen Virol       Date:  1979-08       Impact factor: 3.891

9.  Diagnosis of recent rubella virus infection by demonstration of specific immunoglobulin M antibodies: comparison of solid-phase reverse immunosorbent test with sucrose density gradient centrifugation.

Authors:  G A Denoyel; A Gaspar; D Peyramond
Journal:  J Clin Microbiol       Date:  1981-04       Impact factor: 5.948

10.  Antibody capture radioimmunoassay for anti-rubella IgM.

Authors:  P P Mortimer; R S Tedder; M H Hamblig; M S Shafi; F Burkhardt; U Schilt
Journal:  J Hyg (Lond)       Date:  1981-04
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  5 in total

1.  Comparison of five different methods of rubella IgM antibody testing.

Authors:  H Cubie; E Edmond
Journal:  J Clin Pathol       Date:  1985-02       Impact factor: 3.411

2.  Detection of specific immunoglobulin M antibody to rubella virus by use of an enzyme-labeled antigen.

Authors:  C Bonfanti; O Meurman; P Halonen
Journal:  J Clin Microbiol       Date:  1985-06       Impact factor: 5.948

3.  Public Health Laboratory Service IgM antibody capture enzyme linked immunosorbent assay for detecting rubella specific IgM.

Authors:  K Bellamy; J Hodgson; P S Gardner; P Morgan-Capner
Journal:  J Clin Pathol       Date:  1985-10       Impact factor: 3.411

4.  Analysis of immunoglobulin A antibodies to Helicobacter pylori in serum and gastric juice in relation to mucosal inflammation.

Authors:  S Hayashi; T Sugiyama; K Yokota; H Isogai; E Isogai; K Oguma; M Asaka; N Fujii; Y Hirai
Journal:  Clin Diagn Lab Immunol       Date:  1998-09

5.  Development and evaluation of a capture enzyme-linked immunosorbent assay for determination of rubella immunoglobulin M using monoclonal antibodies.

Authors:  I Gerna; M Zannino; M G Revello; E Petruzzelli; M Dovis
Journal:  J Clin Microbiol       Date:  1987-06       Impact factor: 5.948
  5 in total

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