Literature DB >> 6725422

Release and purification of Trypanosoma brucei variant surface glycoprotein.

G A Cross.   

Abstract

Conditions affecting the solubilization of variant surface glycoprotein (VSG) from Trypanosoma brucei have been investigated. The results obtained form the basis for a convenient and efficient method for VSG purification. VSG release from the cell surface was temperature-dependent, following osmotic lysis at 0 degree C, and was inhibited by low concentrations of Zn2+ but not by tosyl-lysine chloromethyl-ketone (TLCK), phenylmethylsulfonylfluoride (PMSF), or iodoacetamide. These and other results eliminated the possibility that release was due to proteolytic cleavage of the C-terminal hydrophobic tail present on newly synthesized VSG. Bolton and Hunter reagent reacted with several components on living cells.

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Year:  1984        PMID： 6725422     DOI： 10.1002/jcb.240240107

Source DB:  PubMed          Journal:  J Cell Biochem        ISSN： 0730-2312            Impact factor:   4.429


  33 in total

1.  Targeting the variable surface of African trypanosomes with variant surface glycoprotein-specific, serum-stable RNA aptamers.

Authors:  Mihaela Lorger; Markus Engstler; Matthias Homann; H Ulrich Göringer
Journal:  Eukaryot Cell       Date:  2003-02

2.  De novo sphingolipid synthesis is essential for viability, but not for transport of glycosylphosphatidylinositol-anchored proteins, in African trypanosomes.

Authors:  Shaheen S Sutterwala; Caleb H Creswell; Sumana Sanyal; Anant K Menon; James D Bangs
Journal:  Eukaryot Cell       Date:  2007-01-12

3.  Trypanosome variant surface glycoprotein transfer to target membranes: a model for the pathogenesis of trypanosomiasis.

Authors:  M R Rifkin; F R Landsberger
Journal:  Proc Natl Acad Sci U S A       Date:  1990-01       Impact factor: 11.205

4.  Phosphatidylinositol is involved in the attachment of tailed asymmetric acetylcholinesterase to neuronal membranes.

Authors:  M Verdière-Sahuqué; L Garcia; P A Dreyfus; D Goudou; M Nicolet; F Rieger
Journal:  Cell Mol Neurobiol       Date:  1991-02       Impact factor: 5.046

5.  Inhibition of nucleotide sugar transport in Trypanosoma brucei alters surface glycosylation.

Authors:  Li Liu; Yu-Xin Xu; Kacey L Caradonna; Emilia K Kruzel; Barbara A Burleigh; James D Bangs; Carlos B Hirschberg
Journal:  J Biol Chem       Date:  2013-02-26       Impact factor: 5.157

6.  The glycosylphosphatidylinositol-PLC in Trypanosoma brucei forms a linear array on the exterior of the flagellar membrane before and after activation.

Authors:  Orla Hanrahan; Helena Webb; Robert O'Byrne; Elaine Brabazon; Achim Treumann; Jack D Sunter; Mark Carrington; H Paul Voorheis
Journal:  PLoS Pathog       Date:  2009-06-05       Impact factor: 6.823

7.  Distinct donor and acceptor specificities of Trypanosoma brucei oligosaccharyltransferases.

Authors:  Luis Izquierdo; Benjamin L Schulz; João A Rodrigues; Maria Lucia S Güther; James B Procter; Geoffrey J Barton; Markus Aebi; Michael A J Ferguson
Journal:  EMBO J       Date:  2009-07-23       Impact factor: 11.598

8.  Cohesin regulates VSG monoallelic expression in trypanosomes.

Authors:  David Landeira; Jean-Mathieu Bart; Daria Van Tyne; Miguel Navarro
Journal:  J Cell Biol       Date:  2009-07-27       Impact factor: 10.539

9.  The phosphoproteome of bloodstream form Trypanosoma brucei, causative agent of African sleeping sickness.

Authors:  Isabelle R E Nett; David M A Martin; Diego Miranda-Saavedra; Douglas Lamont; Jonathan D Barber; Angela Mehlert; Michael A J Ferguson
Journal:  Mol Cell Proteomics       Date:  2009-04-04       Impact factor: 7.381

10.  Application of electrospray mass spectrometry to the structural determination of glycosylphosphatidylinositol membrane anchors.

Authors:  Isabelle R E Nett; Angela Mehlert; Douglas Lamont; Michael A J Ferguson
Journal:  Glycobiology       Date:  2010-01-24       Impact factor: 4.313
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