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Literature DB >> 6724622

Selective elimination of fibroblasts from cultures of normal human melanocytes.

Abstract

The main obstacle to establishing pure normal human melanocytes in vitro is contamination of the cultures by fibroblasts. The obstacle can be overcome by selective destruction of fibroblasts with geneticin ( G418 sulfate). Treatment of mixed cultures with this drug at a concentration of 100 micrograms/ml for two days results in pure cultures of normal human melanocytes.

Entities: Chemical Species

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Year: 1984 PMID： 6724622 DOI： 10.1007/BF02619590

Source DB: PubMed Journal: In Vitro ISSN： 0073-5655

3 in total

1. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.

Authors: P J Southern; P Berg
Journal: J Mol Appl Genet Date: 1982

2. Selective proliferation of normal human melanocytes in vitro in the presence of phorbol ester and cholera toxin.

Authors: M Eisinger; O Marko
Journal: Proc Natl Acad Sci U S A Date: 1982-03 Impact factor: 11.205

3. Regulation of tyrosinase in human melanocytes grown in culture.

Authors: R Halaban; S H Pomerantz; S Marshall; D T Lambert; A B Lerner
Journal: J Cell Biol Date: 1983-08 Impact factor: 10.539

3 in total

47 in total

1. PIG3V, an immortalized human vitiligo melanocyte cell line, expresses dilated endoplasmic reticulum.

Authors: I C Le Poole; R E Boissy; R Sarangarajan; J Chen; J J Forristal; P Sheth; W Westerhof; G Babcock; P K Das; C B Saelinger
Journal: In Vitro Cell Dev Biol Anim Date: 2000-05 Impact factor: 2.416

Review 2. Aminoglycosides: activity and resistance.

Authors: M P Mingeot-Leclercq; Y Glupczynski; P M Tulkens
Journal: Antimicrob Agents Chemother Date: 1999-04 Impact factor: 5.191

Review 3. Aminoglycoside antibiotics: structure, functions and effects on in vitro plant culture and genetic transformation protocols.

Authors: I M G Padilla; L Burgos
Journal: Plant Cell Rep Date: 2010-07-20 Impact factor: 4.570

4. Isolation and sequence of a cDNA clone for human tyrosinase that maps at the mouse c-albino locus.

Authors: B S Kwon; A K Haq; S H Pomerantz; R Halaban
Journal: Proc Natl Acad Sci U S A Date: 1987-11 Impact factor: 11.205

5. Membranous complexes characteristic of melanocytes derived from patients with Hermansky-Pudlak syndrome type 1 are macroautophagosomal entities of the lysosomal compartment.

Authors: Justin W Smith; Amy Koshoffer; Randal E Morris; Raymond E Boissy
Journal: Pigment Cell Res Date: 2005-12

6. Cell migration and actin organization in cultured human primary, recurrent cutaneous and metastatic melanoma. Time-lapse and image analysis.

Authors: H R Byers; T Etoh; J R Doherty; A J Sober; M C Mihm
Journal: Am J Pathol Date: 1991-08 Impact factor: 4.307

7. New procedure for epidermal cell isolation using kiwi fruit actinidin, and improved culture of melanocytes in the presence of leukaemia inhibitory factor and forskolin.

Authors: Reza Yarani; Kamran Mansouri; Hamid Reza Mohammadi-Motlagh; Mitra Bakhtiari; Ali Mostafaie
Journal: Cell Prolif Date: 2013-05-10 Impact factor: 6.831