Literature DB >> 6721856

Characterization of the ATP-G-actin aggregates formed at low potassium chloride concentration.

E Grazi, A Aleotti, A Ferri.   

Abstract

The ATP-G-actin aggregates formed by incubation of ATP-G-actin in 7.5 mM-KCl were characterized by electron-microscopical observation, by high-pressure liquid chromatography and by the study of the 1,N6-etheno-ATP-ATP exchange reaction between the free and the actin-bound nucleotide. In 30 mM-KCl the initial rate of the reduced-viscosity increase is found to be directly related to the amount of the aggregates formed in the course of the preincubation in 7.5 mM-KCl.

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Year:  1984        PMID: 6721856      PMCID: PMC1153473          DOI: 10.1042/bj2190273

Source DB:  PubMed          Journal:  Biochem J        ISSN: 0264-6021            Impact factor:   3.857


  10 in total

1.  Polymerization of Acanthamoeba actin. Kinetics, thermodynamics, and co-polymerization with muscle actin.

Authors:  D J Gordon; Y Z Yang; E D Korn
Journal:  J Biol Chem       Date:  1976-12-10       Impact factor: 5.157

2.  The kinetics of the exchange of G-actin-bound 1: N6-ethenoadenosine 5'-triphosphate with ATP as followed by fluorescence.

Authors:  F Waechter; J Engel
Journal:  Eur J Biochem       Date:  1975-09-15

3.  Detection of conformational changes in actin by proteolytic digestion: evidence for a new monomeric species.

Authors:  S A Rich; J E Estes
Journal:  J Mol Biol       Date:  1976-07-15       Impact factor: 5.469

4.  Control of rabbit liver fructose-1, 6-diphosphatase activity by magnesium ions.

Authors:  Y Tashima; N Yoshimura
Journal:  J Biochem       Date:  1975-12       Impact factor: 3.387

5.  The regulation of rabbit skeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proteolytic fragments of myosin.

Authors:  J A Spudich; S Watt
Journal:  J Biol Chem       Date:  1971-08-10       Impact factor: 5.157

6.  The polymerization of actin. A study of the nucleation reaction.

Authors:  E Grazi; A Ferri; S Cino
Journal:  Biochem J       Date:  1983-09-01       Impact factor: 3.857

7.  The first step in the polymerisation of actin.

Authors:  J F Rouayrenc; F Travers
Journal:  Eur J Biochem       Date:  1981-05

8.  Mechano-chemical energy transduction in biological systems. The effect of mechanical stimulation on the polymerization of actin: a kinetic study.

Authors:  A Ferri; E Grazi
Journal:  Biochem J       Date:  1982-08-01       Impact factor: 3.857

9.  The primary structure of actin from rabbit skeletal muscle. Completion and analysis of the amino acid sequence.

Authors:  J H Collins; M Elzinga
Journal:  J Biol Chem       Date:  1975-08-10       Impact factor: 5.157

10.  Sonic vibration induces the nucleation of actin in the absence of magnesium ions and cytochalasins inhibit the elongation of the nuclei.

Authors:  K Maruyama
Journal:  J Biol Chem       Date:  1981-02-10       Impact factor: 5.157

  10 in total
  3 in total

1.  An alternative pathway of actin filament elongation. The condensation of small oligomers.

Authors:  E Grazi
Journal:  J Muscle Res Cell Motil       Date:  1989-08       Impact factor: 2.698

2.  Kinetic heterogeneity of F-actin polymers. Further evidence that the elongation reaction may occur through condensation of the actin filaments with small aggregates.

Authors:  E Grazi; E Magri
Journal:  Biochem J       Date:  1987-12-15       Impact factor: 3.857

3.  54Mn2+ as a tracer of the polymerization of actin. Intermediate oligomers condense to give F-actin.

Authors:  E Grazi
Journal:  Biochem J       Date:  1984-11-01       Impact factor: 3.857

  3 in total

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