Prognostic significance of nuclear DNA content in serous ovarian tumors.

Specimens from 73 serous ovarian cancers were examined with respect to DNA content of the tumor cells. The prognostic value of DNA analysis, as reflected in patient survival, was retrospectively compared with that of conventional histological assessment of cancer. DNA in individual tumor cells was measured in sections from the original paraffin-embedded specimens. High proportions of cells with very high DNA values were identified in tumors from 16 patients, 10 of whom died of the disease during the follow-up period. The histological classification was invasive adenocarcinoma in 47 tumors and borderline cancer in 26. All of the patients who died had invasive adenocarcinoma. Although both DNA analysis and histological evaluation were sensitive predictors of mortality, the specificity of the DNA method was distinctly higher (0.90 versus 0.42). The study suggests that analysis of the DNA content of tumor cells can be a useful supplement to histological assessment of cancer and accordingly can significantly assist in the planning of treatment.