Literature DB >> 6712607

Isolation and characterization of glyoxylate dehydrogenase from the fungus Sclerotium rolfsii.

A J Balmforth, A Thomson.   

Abstract

Glyoxylate dehydrogenase (glyoxylate:NAD+ oxidoreductase) was purified 600-fold in three steps from crude extracts of the fungus Sclerotium rolfsii (Corticium rolfsii Curzi). Two of the purification steps involved dye-affinity chromatography. The enzyme is a tetramer of Mr 250 000, with identical subunits of Mr 57 000. Inhibition studies suggest that there is one essential thiol group per active site.

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Year:  1984        PMID： 6712607      PMCID： PMC1153313          DOI： 10.1042/bj2180113

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  9 in total

1.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

2.  The use of Meldolablue in isozyme stains after electrophoresis.

Authors:  V S Turner; D A Hopkinson
Journal:  FEBS Lett       Date:  1979-09-15       Impact factor: 4.124

3.  Anomalous behavior of yeast isocitrate dehydrogenase during isoelectric focusing.

Authors:  J A Illingworth
Journal:  Biochem J       Date:  1972-10       Impact factor: 3.857

4.  Kinetic studies of the acylation of pig muscle D-glyceraldehyde 3-phosphate dehydrogenase by 1,3-diphosphoglycerate and of proton uptake and release in the overall enzyme mechanism.

Authors:  P J Harrigan; D R Trentham
Journal:  Biochem J       Date:  1973-12       Impact factor: 3.857

5.  Octadecanol metabolism in Candida tropicalis.

Authors:  A N Kachhy; A M Madia; V V Modi; N Parekh
Journal:  Indian J Exp Biol       Date:  1972-05       Impact factor: 0.818

6.  The oxidation and reductn of glyoxylate by lactic dehydrogenase.

Authors:  R J Duncan; K F Tipton
Journal:  Eur J Biochem       Date:  1969-11

7.  The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.

Authors:  K Weber; M Osborn
Journal:  J Biol Chem       Date:  1969-08-25       Impact factor: 5.157

8.  The purification of inosine 5'-monophosphate dehydrogenase from Escherichia coli by affinity chromatography on immobilized Procion dyes.

Authors:  C R Lowe; M Hans; N Spibey; W T Drabble
Journal:  Anal Biochem       Date:  1980-05-01       Impact factor: 3.365

9.  Horse liver aldehyde dehydrogenase. Purification and characterization of two isozymes.

Authors:  J Eckfeldt; L Mope; K Takio; T Yonetani
Journal:  J Biol Chem       Date:  1976-01-10       Impact factor: 5.157
  9 in total
  4 in total

1.  Oxalic acid production by citric acid-producing Aspergillus niger overexpressing the oxaloacetate hydrolase gene oahA.

Authors:  Keiichi Kobayashi; Takasumi Hattori; Yuki Honda; Kohtaro Kirimura
Journal:  J Ind Microbiol Biotechnol       Date:  2014-03-11       Impact factor: 3.346

2.  Glyoxylate rather than ascorbate is an efficient precursor for oxalate biosynthesis in rice.

Authors:  Le Yu; Jingzhe Jiang; Chan Zhang; Linrong Jiang; Nenghui Ye; Yusheng Lu; Guozheng Yang; Ee Liu; Changlian Peng; Zhenghui He; Xinxiang Peng
Journal:  J Exp Bot       Date:  2010-03-01       Impact factor: 6.992

3.  Identification and characterization of Sclerotinia sclerotiorum NADPH oxidases.

Authors:  Hyo-jin Kim; Changbin Chen; Mehdi Kabbage; Martin B Dickman
Journal:  Appl Environ Microbiol       Date:  2011-09-02       Impact factor: 4.792

4.  Evidence for a cytoplasmic pathway of oxalate biosynthesis in Aspergillus niger.

Authors:  C P Kubicek; G Schreferl-Kunar; W Wöhrer; M Röhr
Journal:  Appl Environ Microbiol       Date:  1988-03       Impact factor: 4.792
  4 in total

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