Literature DB >> 6712594

Comparison of protein-synthesis rate of alveolar macrophages in vivo and in vitro.

M H Oliver, P J Cole, G J Laurent.   

Abstract

This paper describes and validates a novel method for measuring rates of protein synthesis of rabbit alveolar macrophages in vivo. A rate of 9.3%/day was obtained, compared with 48.9%/day measured in vitro. This study suggests that the procedures involved in the isolation of alveolar macrophages for study in vitro may themselves activate the cell.

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Year:  1984        PMID： 6712594      PMCID： PMC1153279          DOI： 10.1042/bj2170761

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  15 in total

1.  An ultramicro method of amino acid analysis: application to studies of protein metabolism in cultured cells.

Authors:  J Airhart; J Kelley; J E Brayden; R B Low; W S Stirewalt
Journal:  Anal Biochem       Date:  1979-07-01       Impact factor: 3.365

2.  The effect of starvation on the rate of protein synthesis in rat liver and small intestine.

Authors:  M A McNurlan; A M Tomkins; P J Garlick
Journal:  Biochem J       Date:  1979-02-15       Impact factor: 3.857

3.  Protein synthesis in pulmonary alveolar macrophages. Source of amino acids for leucyl-tRNA.

Authors:  J Airhart; J A Arnold; C A Bulman; R B Low
Journal:  Biochim Biophys Acta       Date:  1981-03-26

4.  Anomalous tritium loss in the measurement of tissue hydroxy-[5-3H]proline specific activity following chloramine-T oxidation.

Authors:  G J Laurent; R J McAnulty; M H Oliver
Journal:  Anal Biochem       Date:  1982-07-01       Impact factor: 3.365

5.  Inflammatory and immune processes in the human lung in health and disease: evaluation by bronchoalveolar lavage.

Authors:  G W Hunninghake; J E Gadek; O Kawanami; V J Ferrans; R G Crystal
Journal:  Am J Pathol       Date:  1979-10       Impact factor: 4.307

6.  Assessment of protein turnover in perfused rat liver. Evidence for amino acid compartmentation from differential labeling of free and tRNA-gound valine.

Authors:  E A Khairallah; G E Mortimore
Journal:  J Biol Chem       Date:  1976-03-10       Impact factor: 5.157

7.  Measurement of protein synthesis in rat lungs perfused in situ.

Authors:  C A Watkins; D E Rannels
Journal:  Biochem J       Date:  1980-04-15       Impact factor: 3.857

8.  Prolyl-tRNA-based rates of protein and collagen synthesis in human lung fibroblasts.

Authors:  J N Hildebran; J Airhart; W S Stirewalt; R B Low
Journal:  Biochem J       Date:  1981-08-15       Impact factor: 3.857

9.  Equilibration of leucine between the plasma compartment and leucyl-tRNA in the heart, and turnover of cardiac myosin heavy chain.

Authors:  A W Everett; G Prior; R Zak
Journal:  Biochem J       Date:  1981-01-15       Impact factor: 3.857

10.  Protein turnover in pulmonary macrophages. Utilization of amino acids derived from protein degradation.

Authors:  J A Hammer; D E Rannels
Journal:  Biochem J       Date:  1981-07-15       Impact factor: 3.857
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  3 in total

1.  Pre- and post-natal growth and protein turnover in the lung of the rat.

Authors:  D F Goldspink
Journal:  Biochem J       Date:  1987-02-15       Impact factor: 3.857

2.  Age-dependent changes in porcine alveolar macrophage function during the postnatal period of alveolarization.

Authors:  R Dickie; D R Tasat; E Fernandez Alanis; V Delfosse; A Tsuda
Journal:  Dev Comp Immunol       Date:  2008-09-04       Impact factor: 3.636

3.  Rates of protein turnover in vivo and in vitro in ventricular muscle of hearts from fed and starved rats.

Authors:  V R Preedy; D M Smith; N F Kearney; P H Sugden
Journal:  Biochem J       Date:  1984-09-01       Impact factor: 3.857
  3 in total

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