Literature DB >> 6709829

Use of ascorbic acid in the 51Cr labelling of mouse fibrosarcoma cells.

K A Chaubal, V S Aroskar, C S Godbole, C N Shenoy.   

Abstract

We have studied and discussed the significance of the use of ascorbic acid in 51Cr labelling of cells. The following two advantages seem credible if cells (mouse fibrosarcoma) in culture are incubated for about 2 h in a medium containing Na2 51CrO4 and then ascorbic acid is added to the medium and incubation continued for ten more minutes: (a) the natural intracellular conversion of Cr6+ to Cr3+ appears to be aided, (b) the incorporation of chromium by cells is higher.

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Year:  1984        PMID: 6709829     DOI: 10.1007/bf01326739

Source DB:  PubMed          Journal:  Radiat Environ Biophys        ISSN: 0301-634X            Impact factor:   1.925


  8 in total

1.  The intracellular labeling of Ehrlich mouse ascites tumor cells with radiochromate.

Authors:  P C RAJAM; A L JACKSON; S H BLACK
Journal:  J Lab Clin Med       Date:  1958-05

2.  Survival of blood platelets labeled with chromium.

Authors:  K A AAS; F H GARDNER
Journal:  J Clin Invest       Date:  1958-09       Impact factor: 14.808

3.  Quantitative assay of the lytic action of immune lymphoid cells on 51-Cr-labelled allogeneic target cells in vitro; inhibition by isoantibody and by drugs.

Authors:  K T Brunner; J Mauel; J C Cerottini; B Chapuis
Journal:  Immunology       Date:  1968-02       Impact factor: 7.397

4.  The organ distribution of disseminated 51 Cr-labeled tumor cells.

Authors:  B Fisher; E R Fisher
Journal:  Cancer Res       Date:  1967-02       Impact factor: 12.701

5.  Natural killer (NK) cell activity in the rat. I. Isolation and characterization of the effector cells.

Authors:  C W Reynolds; T Timonen; R B Herberman
Journal:  J Immunol       Date:  1981-07       Impact factor: 5.422

6.  Characterization of natural cytotoxicity in vitro in a spontaneous rat tumor model.

Authors:  M Zöller; S Matzku
Journal:  J Immunol       Date:  1980-04       Impact factor: 5.422

7.  Chromate metabolism in liver microsomes.

Authors:  K W Jennette
Journal:  Biol Trace Elem Res       Date:  1979-03       Impact factor: 3.738

8.  Cytotoxic and clastogenic effects of soluble and insoluble compounds containing hexavalent and trivalent chromium.

Authors:  A G Levis; F Majone
Journal:  Br J Cancer       Date:  1981-08       Impact factor: 7.640

  8 in total

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