Literature DB >> 6708947

Does the concentration of DNA (Co) and the time of incubation (t) as parameters of Cot influence the thermal stability of the DNA duplexes?

D R Bachvarov, I G Ivanov, G G Markov.   

Abstract

It has been shown in a previous paper (8) that the prime product of reassociation of related DNA sequences under open experimental conditions are mismatched duplexes which undergo 'maturation' upon further incubation. Due to this feature, the Tm value of the duplexes of a large number of DNAs is strongly dependent on the Cot value. Here we present data showing that the Tm of the duplexes of such type of DNAs depends also on the concentration of DNA in the range of one and the same Cot value. The significance of this finding in studying the taxonomic relationship by DNA-DNA hybridisation is discussed.

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Year:  1984        PMID： 6708947     DOI： 10.1007/bf00775351

Source DB:  PubMed          Journal:  Mol Biol Rep        ISSN： 0301-4851            Impact factor:   2.316


  13 in total

Review 1.  Hybridization and renaturation kinetics of nucleic acids.

Authors:  J G Wetmur
Journal:  Annu Rev Biophys Bioeng       Date:  1976

2.  A study of the evolution of repeated DNA sequences in primates and the existence of a new class of repetitive sequences in primates.

Authors:  P L Deininger; C W Schmid
Journal:  J Mol Biol       Date:  1979-02-05       Impact factor: 5.469

3.  Significance of nucleic acid hybridization to systematics of antinomycetes.

Authors:  S G Bradley
Journal:  Adv Appl Microbiol       Date:  1975       Impact factor: 5.086

4.  "Maturation" of DNA duplexes.

Authors:  I Ivanov; P Antanov; N Markova; G Markov
Journal:  Mol Biol Rep       Date:  1978-06-16       Impact factor: 2.316

5.  Heterogeneity of Streptomyces DNA.

Authors:  P P Antonov; I G Ivanov; G G Markov
Journal:  FEBS Lett       Date:  1977-07-01       Impact factor: 4.124

6.  Analysis of repeating DNA sequences by reassociation.

Authors:  R J Britten; D E Graham; B R Neufeld
Journal:  Methods Enzymol       Date:  1974       Impact factor: 1.600

Review 7.  New approaches to bacterial taxonomy: perspective and prospects.

Authors:  M Mandel
Journal:  Annu Rev Microbiol       Date:  1969       Impact factor: 15.500

8.  On the heterogeneity of the slow reassociating ("unique") DNA.

Authors:  I G Ivanov; G G Markov
Journal:  Mol Cell Biochem       Date:  1978-06-28       Impact factor: 3.396

9.  Hydroxyapatite column chromatography in procedures for isolation of purified DNA.

Authors:  G G Markov; I G Ivanov
Journal:  Anal Biochem       Date:  1974-06       Impact factor: 3.365

Review 10.  Evolution of higher-organism DNA.

Authors:  D E Kohne
Journal:  Q Rev Biophys       Date:  1970-08       Impact factor: 5.318
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