Literature DB >> 6704378

3-(Trifluoromethyl)-3-(m-[125I]iodophenyl)diazirine, a hydrophobic, photoreactive probe, labels calmodulin and calmodulin fragments in a Ca2+-dependent way.

J Krebs, J Buerkler, D Guerini, J Brunner, E Carafoli.   

Abstract

3-(Trifluoromethyl)-3-(m-[125I]iodophenyl)diazirine [( 125I]TID), a highly hydrophobic, carbene-generating photoreactive probe, labels calmodulin and some of its proteolytic fragments in the Ca2+-bound conformation only. It is assumed that [125I]TID labels hydrophobic sites exposed by the binding of Ca2+. The finding offers a new and powerful means to characterize calmodulin sites that play a role in the interaction with targets.

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Year:  1984        PMID： 6704378     DOI： 10.1021/bi00298a002

Source DB:  PubMed          Journal:  Biochemistry        ISSN： 0006-2960            Impact factor:   3.162


  7 in total

1.  Localization of the membrane-associated region of vesicular stomatitis virus M protein at the N terminus, using the hydrophobic, photoreactive probe 125I-TID.

Authors:  J Lenard; R Vanderoef
Journal:  J Virol       Date:  1990-07       Impact factor: 5.103

2.  Topographic labelling of pore-forming proteins from the outer membrane of Escherichia coli.

Authors:  M G Page; J P Rosenbusch
Journal:  Biochem J       Date:  1986-05-01       Impact factor: 3.857

3.  High-affinity formation of a 2:1 complex between gramicidin S and calmodulin.

Authors:  J A Cox; M Milos; M Comte
Journal:  Biochem J       Date:  1987-09-01       Impact factor: 3.857

4.  Retinoic acid inhibits calmodulin binding to human erythrocyte membranes and reduces membrane Ca2(+)-adenosine triphosphatase activity.

Authors:  F B Davis; T J Smith; M R Deziel; P J Davis; S D Blas
Journal:  J Clin Invest       Date:  1990-06       Impact factor: 14.808

5.  The membrane topology of the amino-terminal domain of the red cell calcium pump.

Authors:  P R Castello; F L González Flecha; A J Caride; H N Fernández; J M Delfino; J P Rossi
Journal:  Protein Sci       Date:  1997-08       Impact factor: 6.725

6.  Crystal structure of calmodulin binding domain of orai1 in complex with Ca2+ calmodulin displays a unique binding mode.

Authors:  Yanshun Liu; Xunhai Zheng; Geoffrey A Mueller; Mack Sobhany; Eugene F DeRose; Yingpei Zhang; Robert E London; Lutz Birnbaumer
Journal:  J Biol Chem       Date:  2012-10-29       Impact factor: 5.157

7.  Photolabeling evidence for calcium-induced conformational changes at the ATP binding site of scallop myosin.

Authors:  B A Kerwin; R G Yount
Journal:  Proc Natl Acad Sci U S A       Date:  1993-01-01       Impact factor: 11.205
  7 in total

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