Isolation of neurosecretory granules from the neurohaemal areas of peptidergic systems of Lymnaea stagnalis, with special reference to the ovulation hormone-producing caudodorsal cells.

Neurosecretory granules (NSG) were isolated by density-gradient centrifugation from homogenates of the intercerebral commissure (COM) and the median lip nerves (LN) of the freshwater snail Lymnaea stagnalis. COM and LN are rich in axon terminals of the ovulation hormone-producing caudodorsal cells (CDC) and of the growth hormone-producing light green cells (LGC), respectively. Electron microscopy of COM isolates showed that a single fraction contained large numbers of NSG, which were identical to NSG of CDC axon terminals of the intact COM. High ovulation hormone activity was associated with this fraction. LN NSG were also isolated in large numbers in a single fraction. The isolated LN NSG were identical to NSG of intact LGC axon terminals. They appeared to have a higher specific gravity than CDC NSG.