Literature DB >> 669825

Application of charge shift electrophoresis to antigenic analysis of mycoplasmic membranes by two-dimensional (crossed) immunoelectrophoresis.

A G Alexander, G E Kenny.   

Abstract

Both the anodic migration and resolution of membrane antigens of Mycoplasma arginini were greatly improved in two-dimensional immunoelectrophoresis by incorporating both a neutral detergent, Triton X-100, and an anionic detergent, sodium deoxycholate, in the agarose support for first phase electrophoresis. The mobility of cytoplasmic antigens was unaffected at concentrations of 0.1% or less of sodium deoxycholate.

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Year:  1978        PMID： 669825      PMCID： PMC421938          DOI： 10.1128/iai.20.3.861-863.1978

Source DB:  PubMed          Journal:  Infect Immun        ISSN： 0019-9567            Impact factor:   3.441


  8 in total

1.  Detection of amphiphilic proteins and peptides in complex mixtures. Charge-shift crossed immunoelectrophoresis and two-dimensional charge-shift electrophoresis.

Authors:  S Bhakdi; B Bhakdi-Lehnen; O J Bjerrum
Journal:  Biochim Biophys Acta       Date:  1977-10-03

2.  Localization of the Tween 20-soluble membrane proteins of Acholeplasma laidlawii by crossed immunoelectrophoresis.

Authors:  K E Johansson; S Hjertén
Journal:  J Mol Biol       Date:  1974-06-25       Impact factor: 5.469

3.  Antigenic analysis of Chlamydiae by two-dimensional immunoelectrophoresis. I. Antigenic heterogeneity between C. trachomatis and C. psittaci.

Authors:  H D Caldwell; C C Kuo; G E Kenny
Journal:  J Immunol       Date:  1975-10       Impact factor: 5.422

4.  Antigenic and enzymatic architecture of Micrococcus lysodeikticus membranes established by crossed immunoelectrophoresis.

Authors:  P Owen; M R Salton
Journal:  Proc Natl Acad Sci U S A       Date:  1975-09       Impact factor: 11.205

5.  Antigenic analysis of Neisseria gonorrhoeae by crossed immunoelectrophoresis.

Authors:  C J Smyth; A E Friedman-Kien; M R Salton
Journal:  Infect Immun       Date:  1976-04       Impact factor: 3.441

6.  Serological comparison of five arginine-utilizing Mycoplasma species by two-dimensional immunoelectrophoresis.

Authors:  C E Thirkill; G E Kenny
Journal:  Infect Immun       Date:  1974-09       Impact factor: 3.441

7.  Characterization of membrane and cytoplasmic antigens of Mycoplasma arginini by two-dimensional (crossed) immunoelectrophoresis.

Authors:  A G Alexander; G E Kenny
Journal:  Infect Immun       Date:  1977-01       Impact factor: 3.441

8.  Charge shift electrophoresis: simple method for distinguishing between amphiphilic and hydrophilic proteins in detergent solution.

Authors:  A Helenius; K Simons
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205
  8 in total
  6 in total

1.  Immunochemical analysis of triton X-100-insoluble residues from Micrococcus lysodeikticus membranes.

Authors:  P Owen; H Doherty
Journal:  J Bacteriol       Date:  1979-12       Impact factor: 3.490

2.  Immunochemical characterization of a heat-stable surface antigen of Mycoplasma pulmonis expressing both species-specific and strain-specific determinants.

Authors:  E J Nichols; G E Kenny
Journal:  Infect Immun       Date:  1984-05       Impact factor: 3.441

3.  Identification and characterization of a major surface antigen of Giardia lamblia.

Authors:  D A Einfeld; H H Stibbs
Journal:  Infect Immun       Date:  1984-11       Impact factor: 3.441

4.  Polypeptides and functions of antigens from human coronaviruses 229E and OC43.

Authors:  O W Schmidt; G E Kenny
Journal:  Infect Immun       Date:  1982-02       Impact factor: 3.441

5.  Antigenic analysis of Legionella pneumophila and Tatlockia micdadei (Legionella micdadei) by two-dimensional (crossed) immunoelectrophoresis.

Authors:  J R Joly; G E Kenny
Journal:  Infect Immun       Date:  1982-02       Impact factor: 3.441

6.  Characterization of the strain-specific and common surface antigens of Mycoplasma arginini.

Authors:  A G Alexander; G E Kenny
Journal:  Infect Immun       Date:  1980-08       Impact factor: 3.441
  6 in total

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