Literature DB >> 6697392

Circles with two tandem LTRs are precursors to integrated retrovirus DNA.

A T Panganiban, H M Temin.   

Abstract

Infection of susceptible cells by retroviruses results in the synthesis of linear DNA with two long terminal repeats (LTRs), circular DNA with a single LTR, and circular DNA with two tandem LTRs. To determine which of these unintegrated molecules serves as the precursor to the provirus, we inserted into a retrovirus vector a 49 bp fragment containing the junction formed by in vivo blunt-end ligation of two LTRs. Infection of chicken embryo fibroblasts with virus recovered from this vector and subsequent characterization of the proviral DNA revealed that efficient integration can occur from this introduced junction sequence. Therefore, circular DNA with two tandem LTRs is a precursor to the provirus.

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Year:  1984        PMID： 6697392     DOI： 10.1016/0092-8674(84)90347-7

Source DB:  PubMed          Journal:  Cell        ISSN： 0092-8674            Impact factor:   41.582


  53 in total

1.  Multiple complex families of endogenous retroviruses are highly conserved in the genus Gallus.

Authors:  M T Boyce-Jacino; K O'Donoghue; A J Faras
Journal:  J Virol       Date:  1992-08       Impact factor: 5.103

2.  MuA-mediated in vitro cloning of circular DNA: transpositional autointegration and the effect of MuB.

Authors:  Elsi Pulkkinen; Saija Haapa-Paananen; Harri Savilahti
Journal:  Mol Genet Genomics       Date:  2016-02-04       Impact factor: 3.291

3.  Circles with two tandem long terminal repeats are specifically cleaved by pol gene-associated endonuclease from avian sarcoma and leukosis viruses: nucleotide sequences required for site-specific cleavage.

Authors:  G Duyk; M Longiaru; D Cobrinik; R Kowal; P deHaseth; A M Skalka; J Leis
Journal:  J Virol       Date:  1985-11       Impact factor: 5.103

4.  Structure of a cloned circular retroviral DNA containing a tRNA sequence between the terminal repeats.

Authors:  J Colicelli; S P Goff
Journal:  J Virol       Date:  1986-02       Impact factor: 5.103

5.  Proteolytic processing of avian sarcoma and leukosis viruses pol-endo recombinant proteins reveals another pol gene domain.

Authors:  F Alexander; J Leis; D A Soltis; R M Crowl; W Danho; M S Poonian; Y C Pan; A M Skalka
Journal:  J Virol       Date:  1987-02       Impact factor: 5.103

6.  Retroviruses and evolution.

Authors:  H M Temin
Journal:  Cell Biophys       Date:  1986-12

7.  The termini of extrachromosomal linear copia elements.

Authors:  A J Flavell; C Brierley
Journal:  Nucleic Acids Res       Date:  1986-05-12       Impact factor: 16.971

8.  Measurement of human immunodeficiency virus type 1 preintegration transcription by using Rev-dependent Rev-CEM cells reveals a sizable transcribing DNA population comparable to that from proviral templates.

Authors:  Subashini R Iyer; Dongyang Yu; Angélique Biancotto; Leonid B Margolis; Yuntao Wu
Journal:  J Virol       Date:  2009-06-24       Impact factor: 5.103

9.  The avian retroviral integration protein cleaves the terminal sequences of linear viral DNA at the in vivo sites of integration.

Authors:  M Katzman; R A Katz; A M Skalka; J Leis
Journal:  J Virol       Date:  1989-12       Impact factor: 5.103

Review 10.  Retroviral Integrase: Then and Now.

Authors:  Mark D Andrake; Anna Marie Skalka
Journal:  Annu Rev Virol       Date:  2015-11       Impact factor: 10.431
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