Literature DB >> 669727

Cell cycle analysis and synchronization of the TN-368 insect cell line.

D E Lynn, W F Hink.   

Abstract

A cell cycle analysis of the Trichoplusia ni (TN-368) insect cell line is described. By means of autoradiography and percent labeled metaphase data, the cell cycle parameters were determined to be as follows: S, 4.5 hr; G2, 8.5 hr; M, 0.5 hr; G1, 1.0 hr; the total cell time being 14.5 hr. A synchronization procedure using 50 mM thymidine in a double block procedure was used to provide a method of obtaining a large number of cells in particular cell cycle phases, especially S and G2.

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Year:  1978        PMID： 669727     DOI： 10.1007/BF02618228

Source DB:  PubMed          Journal:  In Vitro        ISSN： 0073-5655


  6 in total

1.  Cell population kinetics in the intestinal epithelium of the mouse.

Authors:  H QUASTLER; F G SHERMAN
Journal:  Exp Cell Res       Date:  1959-06       Impact factor: 3.905

2.  Phasing, Mitotic Delay, and Chromosomal Aberrations in Mammalian Cells.

Authors:  T T Puck
Journal:  Science       Date:  1964-05-01       Impact factor: 47.728

3.  Preparation of large quantities of synchronized mammalian cells in late G1 in the pre-DNA replicative phase of the cell cycle.

Authors:  R A Tobey; H A Crissman
Journal:  Exp Cell Res       Date:  1972-12       Impact factor: 3.905

4.  The cell cycle of an established line of Drosophila melanogaster cells in vitro.

Authors:  S Dolfini; A M Courgeon; L Tiepolo
Journal:  Experientia       Date:  1970-09-26

5.  A method for synchronization of animal cells in culture.

Authors:  T Ooka
Journal:  Methods Cell Biol       Date:  1976       Impact factor: 1.441

6.  Established insect cell line from the cabbage looper, Trichoplusia ni.

Authors:  W F Hink
Journal:  Nature       Date:  1970-05-02       Impact factor: 49.962
  6 in total
  3 in total

1.  Cell cycle parameters in Aedes albopictus mosquito cells.

Authors:  A Gerenday; A M Fallon
Journal:  In Vitro Cell Dev Biol Anim       Date:  1996-05       Impact factor: 2.416

2.  Effects of fetal bovine serum deprivation in cell cultures on the production of Anticarsia gemmatalis multinucleopolyhedrovirus.

Authors:  Diego L Mengual Gómez; Mariano N Belaich; Vanina A Rodríguez; Pablo D Ghiringhelli
Journal:  BMC Biotechnol       Date:  2010-09-15       Impact factor: 2.563

3.  Genome sequence of Perigonia lusca single nucleopolyhedrovirus: insights into the evolution of a nucleotide metabolism enzyme in the family Baculoviridae.

Authors:  Daniel M P Ardisson-Araújo; Rayane Nunes Lima; Fernando L Melo; Rollie J Clem; Ning Huang; Sônia Nair Báo; Daniel R Sosa-Gómez; Bergmann M Ribeiro
Journal:  Sci Rep       Date:  2016-06-07       Impact factor: 4.379
  3 in total

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