Literature DB >> 6696723

Cell-free translation of messenger RNA for chondroitin sulphate proteoglycan core protein in rat cartilage.

B M Vertel, W B Upholt, A Dorfman.   

Abstract

Total RNA was extracted from the cartilage tissues rat Swarm chondrosarcoma, neonatal-rat breastplate and embryonic-chicken sterna and translated in wheat-germ cell-free reactions. The core protein of the chondroitin sulphate proteoglycan subunit was identified among translation products of rat mRNA by its apparent Mr of 330 000 and by its immunoprecipitation with specific antisera prepared against rat or chicken proteoglycan antigens. The apparent Mr of the rat proteoglycan core protein is 8000-10000 less than that of the equivalent chicken cartilage core-protein product.

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Year:  1984        PMID： 6696723      PMCID： PMC1153204          DOI： 10.1042/bj2170259

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  26 in total

1.  Isolation and translation of calvaria procollagen messenger ribonucleic acids.

Authors:  H Boedtker; A M Frischauf; H Lehrach
Journal:  Biochemistry       Date:  1976-11-02       Impact factor: 3.162

2.  Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.

Authors:  R A Laskey; A D Mills
Journal:  Eur J Biochem       Date:  1975-08-15

3.  Levels of translatable mRNAs for cell surface protein, collagen precursors, and two membrane proteins are altered in Rous sarcoma virus-transformed chick embryo fibroblasts.

Authors:  S L Adams; M E Sobel; B H Howard; K Olden; K M Yamada; B de Crombrugghe; I Pastan
Journal:  Proc Natl Acad Sci U S A       Date:  1977-08       Impact factor: 11.205

4.  Biosynthesis of chondroitin 4-sulfate-proteoglycan by a transplantable rat chondrosarcoma.

Authors:  A C Stoolmiller; N B Schwartz; A Dorfman
Journal:  Arch Biochem Biophys       Date:  1975-11       Impact factor: 4.013

Review 5.  Glycosaminoglycans and their binding to biological macromolecules.

Authors:  U Lindahl; M Höök
Journal:  Annu Rev Biochem       Date:  1978       Impact factor: 23.643

6.  Isolation and characterization of a collagen from chick cartilage containing three identical alpha chains.

Authors:  E J Miller
Journal:  Biochemistry       Date:  1971-04-27       Impact factor: 3.162

7.  Solvent-dependent changes in proteoglycan subunit conformation in aqueous guanidine hydrochloride solutions.

Authors:  S G Pasternack; A Veis; M Breen
Journal:  J Biol Chem       Date:  1974-04-10       Impact factor: 5.157

8.  Swarm rat chondrosarcoma proteoglycans. Purification of aggregates by zonal centrifugation of preformed cesium sulfate gradients.

Authors:  K Kimata; J H Kimura; E J Thonar; H J Barrach; S I Rennard; V C Hascall
Journal:  J Biol Chem       Date:  1982-04-10       Impact factor: 5.157

9.  The link proteins as specific components of cartilage proteoglycan aggregates in vivo. Associative extraction of proteoglycan aggregate from swarm rat chondrosarcoma.

Authors:  B Caterson; J R Baker
Journal:  J Biol Chem       Date:  1979-04-10       Impact factor: 5.157

10.  Isolation and characterization of proteoglycans from the swarm rat chondrosarcoma.

Authors:  T R Oegema; V C Hascall; D D Dziewiatkowski
Journal:  J Biol Chem       Date:  1975-08-10       Impact factor: 5.157
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  2 in total

1.  The relation of RNA synthesis to chondroitin sulphate biosynthesis in cultured bovine cartilage.

Authors:  D J McQuillan; C J Handley; H C Robinson; K Ng; C Tzaicos
Journal:  Biochem J       Date:  1986-04-15       Impact factor: 3.857

2.  Proteoglycan biosynthesis in chondrocytes: protein A-gold localization of proteoglycan protein core and chondroitin sulfate within Golgi subcompartments.

Authors:  A Ratcliffe; P R Fryer; T E Hardingham
Journal:  J Cell Biol       Date:  1985-12       Impact factor: 10.539
  2 in total

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