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Literature DB >> 6694743

Effect of chromosomal position on amplification of transfected genes in animal cells.

G M Wahl, B Robert de Saint Vincent, M L DeRose.

Abstract

A single protein (CAD) contains the first three enzymatic activities of de novo uridine biosynthesis. The chromosomal location of CAD genes introduced into Chinese hamster ovary cells significantly affects the frequency and cytogenetic result of their amplification. The amplification frequency in one transformant is 100-fold that of the others; in another, amplification of donated genes inserted near a centromere results in chromosome instability and rearrangements.

Entities: Chemical Gene Species

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Year: 1984 PMID： 6694743 DOI： 10.1038/307516a0

Source DB: PubMed Journal: Nature ISSN： 0028-0836 Impact factor: 49.962

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Cited

34 in total

1. Elements which stimulate gene amplification in mammalian cells: role of recombinogenic sequences/structures and transcriptional activation.

Authors: J G McArthur; L K Beitel; J W Chamberlain; C P Stanners
Journal: Nucleic Acids Res Date: 1991-05-11 Impact factor: 16.971

2. Replication timing control can be maintained in extrachromosomally amplified genes.

Authors: S M Carroll; J Trotter; G M Wahl
Journal: Mol Cell Biol Date: 1991-09 Impact factor: 4.272

3. Oncogenes result in genomic alterations that activate a transcriptionally silent, dominantly selectable reporter gene (neo).

Authors: R E Drews; V T Chan; L E Schnipper
Journal: Mol Cell Biol Date: 1992-01 Impact factor: 4.272

9. Coamplification and coexpression of human tissue-type plasminogen activator and murine dihydrofolate reductase sequences in Chinese hamster ovary cells.

Authors: R J Kaufman; L C Wasley; A J Spiliotes; S D Gossels; S A Latt; G R Larsen; R M Kay
Journal: Mol Cell Biol Date: 1985-07 Impact factor: 4.272

10. Escherichia coli aspartate transcarbamylase: a novel marker for studies of gene amplification and expression in mammalian cells.

Authors: J C Ruiz; G M Wahl
Journal: Mol Cell Biol Date: 1986-09 Impact factor: 4.272

Effect of chromosomal position on amplification of transfected genes in animal cells.

1. Elements which stimulate gene amplification in mammalian cells: role of recombinogenic sequences/structures and transcriptional activation.

2. Replication timing control can be maintained in extrachromosomally amplified genes.

3. Oncogenes result in genomic alterations that activate a transcriptionally silent, dominantly selectable reporter gene (neo).

4. Chromosomal destabilization during gene amplification.

5. Complex chromosomal neighborhood effects determine the adaptive potential of a gene under selection.

6. Preferential amplification of rearranged sequences near amplified adenylate deaminase genes.

7. Genomic organization of low copy number sequences that are associated with deca-satellite DNA in the monkey genome.

8. Molecular characterization of ataxia telangiectasia T cell clones. III. Mapping the 14q32.1 distal breakpoint.

9. Coamplification and coexpression of human tissue-type plasminogen activator and murine dihydrofolate reductase sequences in Chinese hamster ovary cells.

10. Escherichia coli aspartate transcarbamylase: a novel marker for studies of gene amplification and expression in mammalian cells.