High affinity binding of the mastoparans by calmodulin.

Calmodulin exhibits high affinity, calcium-dependent binding of the mastoparans--a group of cytoactive tetradecapeptides. The dissociation constants for the peptide-calmodulin complexes determined in 0.20 N KCl, 1.0 mM CaCl2, pH 7.3 are approximately 0.3 nM for mastoparan, approximately 0.9 nM for mastoparan X, and approximately 3.5 nM for Polistes mastoparan. The dissociation constant for the mastoparan-calmodulin complex is the smallest known for any calmodulin binding protein or peptide, suggesting that some type of peptide-calmodulin interaction could be physiologically significant.