Literature DB >> 6688290

Synthesis and characterization of a DNA complementary to Xenopus laevis albumin mRNA.

G J Dimitriadis, V Zongza-Dimitriadis.   

Abstract

Albumin complementary DNA (cDNA) was transcribed from purified albumin mRNA from the liver of Xenopus laevis. The resultant cDNA was an almost full length copy as defined by denaturing gel electrophoresis; was hybridized specifically to albumin mRNA with pseudo-first-order reaction kinetics and the mRNA . cDNA hybrid exhibited a sharp melting profile with a Tm of 83 degrees C. The identity of the cDNA was confirmed by gel electrophoresis following hybridization-arrested translation.

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Year:  1983        PMID： 6688290     DOI： 10.1007/bf00224922

Source DB:  PubMed          Journal:  Mol Cell Biochem        ISSN： 0300-8177            Impact factor:   3.396


  21 in total

Review 1.  Steroid receptors: elements for modulation of eukaryotic transcription.

Authors:  K R Yamamoto; B M Alberts
Journal:  Annu Rev Biochem       Date:  1976       Impact factor: 23.643

2.  Parenchymal cells purified from Xenopus liver and maintained in primary culture synthesize vitellogenin in response to estradiol-17 beta and serum albumin in response to dexamethasone.

Authors:  L J Wangh; J A Osborne; C C Hentschel; R Tilly
Journal:  Dev Biol       Date:  1979-06       Impact factor: 3.582

3.  Serum albumin production by hepatoma cells in culture: direct evidence for stimulation by hydrocortisone.

Authors:  F C Bancroft; L Levine; A H Tashjian
Journal:  Biochem Biophys Res Commun       Date:  1969-12-04       Impact factor: 3.575

4.  A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.

Authors:  W M Bonner; R A Laskey
Journal:  Eur J Biochem       Date:  1974-07-01

5.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

6.  Synthesis of angiotensinogen by isolated rat liver cells and its regulation in comparison to serum albumin.

Authors:  K Weigand; H Warnze; C Falge
Journal:  Biochem Biophys Res Commun       Date:  1977-03-07       Impact factor: 3.575

7.  An efficient mRNA-dependent translation system from reticulocyte lysates.

Authors:  H R Pelham; R J Jackson
Journal:  Eur J Biochem       Date:  1976-08-01

8.  Synthesis of full length cDNAs from four partially purified oviduct mRNAs.

Authors:  G N Buell; M P Wickens; F Payvar; R T Schimke
Journal:  J Biol Chem       Date:  1978-04-10       Impact factor: 5.157

9.  Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.

Authors:  G K McMaster; G G Carmichael
Journal:  Proc Natl Acad Sci U S A       Date:  1977-11       Impact factor: 11.205

10.  Isolation and characterization of xenopus laevis albumin mRNA.

Authors:  G J Dimitriadis
Journal:  Eur J Biochem       Date:  1981-08
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  1 in total

1.  Effect of estrogen on Xenopus laevis albumin mRNA levels.

Authors:  V Zongza-Dimitriadis; G J Dimitriadis
Journal:  Mol Cell Biochem       Date:  1984-09       Impact factor: 3.396
  1 in total

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