Literature DB >> 6687150

Growth and oxygen requirements of antibody producing mouse hybridoma cells in suspension culture.

R Boraston, P W Thompson, S Garland, J R Birch.   

Abstract

Mouse hybridoma cells producing a monoclonal antibody to anti-B blood group antigen have been grown in airlift fermenters up to 30 litres capacity. As a prerequisite for scaling the process up, the oxygen utilization rate of the cells has been established by a dynamic method using an oxygen electrode. The data is compared with that obtained from a chemostat under oxygen limited steady state conditions.

Entities:  

Mesh:

Year:  1983        PMID: 6687150

Source DB:  PubMed          Journal:  Dev Biol Stand        ISSN: 0301-5149


  14 in total

1.  Comparison of specific rates of hybridoma growth and metabolism in batch and continuous cultures.

Authors:  J L Goergen; A Marc; J M Engasser
Journal:  Cytotechnology       Date:  1992       Impact factor: 2.058

2.  Specific monoclonal antibody productivity and the cell cycle-comparisons of batch, continuous and perfusion cultures.

Authors:  M al-Rubeai; A N Emery; S Chalder; D C Jan
Journal:  Cytotechnology       Date:  1992       Impact factor: 2.058

3.  Methods for determination of growth-rate-dependent changes in hybridoma volume, shape and surface structure during continuous recycle.

Authors:  N K Goebel; R Kuehn; M C Flickinger
Journal:  Cytotechnology       Date:  1990-07       Impact factor: 2.058

4.  Batch production and growth kinetics of hybridomas.

Authors:  O W Merten
Journal:  Cytotechnology       Date:  1988-02       Impact factor: 2.058

5.  Influence of bcl-2 on antibody productivity in high cell density perfusion cultures of hybridoma.

Authors:  D Fassnacht; S Rössing; R P Singh; M Al-Rubeai; R Pörtner
Journal:  Cytotechnology       Date:  1999-07       Impact factor: 2.058

6.  Variable functions of bcl-2 in mediating bioreactor stress- induced apoptosis in hybridoma cells.

Authors:  A Perani; R P Singh; R Chauhan; M Al-Rubeai
Journal:  Cytotechnology       Date:  1998-11       Impact factor: 2.058

7.  Reactor design for large scale suspension animal cell culture.

Authors:  J Varley; J Birch
Journal:  Cytotechnology       Date:  1999-05       Impact factor: 2.058

8.  The influence of dissolved oxygen tension on the metabolic activity of an immobilized hybridoma population.

Authors:  J Thömmes; J Gätgens; M Biselli; P W Runstadler; C Wandrey
Journal:  Cytotechnology       Date:  1993       Impact factor: 2.058

9.  Metabolic and kinetic studies of hybridomas in exponentially fed-batch cultures using T-flasks.

Authors:  A E Higareda; L D Possani; O T Ramírez
Journal:  Cytotechnology       Date:  1994       Impact factor: 2.058

10.  Agitation, aeration and perfusion modules for cell culture bioreactors.

Authors:  C Fenge; C Klein; C Heuer; U Siegel; E Fraune
Journal:  Cytotechnology       Date:  1993       Impact factor: 2.058

View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.