Literature DB >> 6685223

Influence of protein net charge on the nucleic acid helix-destabilizing activity of various pancreatic ribonucleases.

A Carsana, A Furia, M Libonati.   

Abstract

Helix-destabilization of double-stranded poly[d(A-T)]induced by various homologous pancreatic ribonucleases which differ in their net charges has been studied under different ionic strength conditions. The response of the destabilizing activity of the various proteins to ionic strength is represented by bell-shaped curves, whose maxima are shifted to higher ionic strength values the higher the number of positive charges of the RNAase involved in the nucleic acid-protein complex. This observation is discussed, and a model proposed, that could explain the experimental results presented.

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Year:  1983        PMID： 6685223     DOI： 10.1007/bf00228773

Source DB:  PubMed          Journal:  Mol Cell Biochem        ISSN： 0300-8177            Impact factor:   3.396


  17 in total

1.  Degradation of DNA . RNA hybrids by aggregates of pancreatic ribonuclease.

Authors:  M Libonati; S Sorrentino; R Galli; R La Montagna; A Di Donato
Journal:  Biochim Biophys Acta       Date:  1975-10-15

2.  THE DESTABILIZING EFFECT OF RIBONUCLEASE ON THE HELICAL DNA STRUCTURE.

Authors:  G FELSENFELD; G SANDEEN; P H VONHIPPEL
Journal:  Proc Natl Acad Sci U S A       Date:  1963-10       Impact factor: 11.205

3.  The isolation and characterization of ribonucleases from sheep pancreas.

Authors:  S E AQVIST; C B ANFINSEN
Journal:  J Biol Chem       Date:  1959-05       Impact factor: 5.157

4.  The primary structure of goat and sheep pancreatic ribonucleases.

Authors:  G W Welling; A J Scheffer; J J Beintema
Journal:  FEBS Lett       Date:  1974-04-15       Impact factor: 4.124

Review 5.  [Active sites of enzymes: stereochemistry and dynamics].

Authors:  M Ia Karpeĭskiĭ
Journal:  Mol Biol (Mosk)       Date:  1976 Nov-Dec

Review 6.  Double-stranded RNA.

Authors:  M Libonati; A Carsana; A Furia
Journal:  Mol Cell Biochem       Date:  1980-08-16       Impact factor: 3.396

7.  Ionic control of enzymic degradation of double-stranded RNA.

Authors:  S Sorrentino; A Carsana; A Furia; J Doskocil; M Libonati
Journal:  Biochim Biophys Acta       Date:  1980-08-26

8.  Kinetics of the action of papain on fluorescent peptide substrates.

Authors:  J A Mattis; J S Fruton
Journal:  Biochemistry       Date:  1976-05-18       Impact factor: 3.162

9.  Involvement of basic amino acids in the activity of a nucleic acid helix-destabilizing protein.

Authors:  R L Karpel; D J Merkler; B K Flowers; M D Delahunty
Journal:  Biochim Biophys Acta       Date:  1981-06-26

10.  Primary structure of pronghorn pancreatic ribonuclease: close relationship between giraffe and pronghorn.

Authors:  J J Beintema; W Gaastra; J Munniksma
Journal:  J Mol Evol       Date:  1979-11       Impact factor: 2.395
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  4 in total

1.  Structural properties of trimers and tetramers of ribonuclease A.

Authors:  A Nenci; G Gotte; M Bertoldi; M Libonati
Journal:  Protein Sci       Date:  2001-10       Impact factor: 6.725

2.  The activity on double-stranded RNA of aggregates of ribonuclease A higher than dimers increases as a function of the size of the aggregates.

Authors:  M Libonati; M Bertoldi; S Sorrentino
Journal:  Biochem J       Date:  1996-08-15       Impact factor: 3.857

Review 3.  Oligomerization of bovine ribonuclease A: structural and functional features of its multimers.

Authors:  Massimo Libonati; Giovanni Gotte
Journal:  Biochem J       Date:  2004-06-01       Impact factor: 3.857

4.  Revisiting the action of bovine ribonuclease A and pancreatic-type ribonucleases on double-stranded RNA.

Authors:  M Libonati; S Sorrentino
Journal:  Mol Cell Biochem       Date:  1992-11-18       Impact factor: 3.396
  4 in total

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