Literature DB >> 6684422

Identification of ecdysonoic acid and 20-hydroxyecdysonoic acid isolated from developing eggs of Schistocerca gregaria and pupae of Spodoptera littoralis.

R E Isaac, N P Milner, H H Rees.   

Abstract

Ecdysonoic acid and 20-hydroxyecdysonoic acid have been purified from developing eggs of the desert locust, Schistocerca gregaria, by high performance liquid chromatography (h.p.l.c.), and their structures were determined by p.m.r. spectroscopy and fast atom bombardment mass spectrometry of the free and methyl ester derivatives. 20-Hydroxyecdysonoic acid was also characterized from Spodoptera littoralis pupae. The occurrence of both 20-hydroxyecdysonoic acid and ecdysonoic acid in Sp. littoralis pupae was also established by h.p.l.c. comparison of the 3H-labelled acids formed from [3H]ecdysone and of their methyl esters with the corresponding substances from Sch. gregaria. The significance of ecdysteroid acids as products of ecdysteroid inactivation is discussed.

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Year:  1983        PMID： 6684422      PMCID： PMC1152117          DOI： 10.1042/bj2130261

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  2 in total

1.  Sulphage esters as inactivation products of ecdysone in Locusta migratoria.

Authors:  J Koolman; J A Hoffmann; P Karlson
Journal:  Hoppe Seylers Z Physiol Chem       Date:  1973-09

2.  Biosynthesis and inactivation of ecdysone during the pupal-adult development of the cabbage butterfly, Pieris brassicae L.

Authors:  P Beydon; J Claret; P Porcheron; R Lafont
Journal:  Steroids       Date:  1981-12       Impact factor: 2.668
  2 in total
  5 in total

Review 1.  Fast atom bombardment mass spectrometry and its application to the analysis of some peptides and proteins.

Authors:  M E Hemling
Journal:  Pharm Res       Date:  1987-02       Impact factor: 4.200

2.  Induction of an inactivation pathway for ecdysteroids in larvae of the cotton leafworm, Spodoptera littoralis.

Authors:  J H Chen; M Kabbouh; M J Fisher; H H Rees
Journal:  Biochem J       Date:  1994-07-01       Impact factor: 3.857

3.  Isolation and identification of 3-acetylecdysone 2-phosphate, a metabolite of ecdysone, from developing eggs of Schistocerca gregaria.

Authors:  R E Isaac; H P Desmond; H H Rees
Journal:  Biochem J       Date:  1984-01-01       Impact factor: 3.857

4.  Isolation and identification of ecdysteroid phosphates and acetylecdysteroid phosphates from developing eggs of the locust, Schistocerca gregaria.

Authors:  R E Isaac; H H Rees
Journal:  Biochem J       Date:  1984-07-15       Impact factor: 3.857

5.  Identification of the 22-phosphate esters of ecdysone, 2-deoxyecdysone, 20-hydroxyecdysone and 2-deoxy-20-hydroxyecdysone from newly laid eggs of the desert locust, Schistocerca gregaria.

Authors:  R E Isaac; M E Rose; H H Rees; T W Goodwin
Journal:  Biochem J       Date:  1983-08-01       Impact factor: 3.857
  5 in total

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