Literature DB >> 6682971

Isolation of the messenger RNA for 8S DNA ligase in early developing axolotl egg and its cell free translation.

P Thiebaud, J Lefresne, J Signoret, J C David.   

Abstract

A new DNA ligase activity is expressed when the Axolotl eggs enter cleavage. The messenger RNA can be labelled by [3H] uridine thereby indicating its de novo synthesis. This new genetic expression is occurring just before cleavage and is the earliest found during Amphibian development. The newly synthesized [3H] mRNA can be translated in vitro in the rabbit reticulocyte lysate system. The resulting product is a 160 K protein specifically immunoprecipitated with the antiserum directed against 8S DNA ligase. This in vitro translated polypeptide exhibits 8S DNA ligase activity specific of activated or fertilized eggs but does not display 6S DNA ligase activity of non activated eggs.

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Year:  1983        PMID： 6682971      PMCID： PMC325908          DOI： 10.1093/nar/11.9.2563

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  23 in total

1.  Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography.

Authors:  R A Laskey; A D Mills
Journal:  Eur J Biochem       Date:  1975-08-15

2.  Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undergraded polysomes and messenger ribonucleic acid.

Authors:  R D Palmiter
Journal:  Biochemistry       Date:  1974-08-13       Impact factor: 3.162

3.  Mechanism of DNA chain growth. I. Possible discontinuity and unusual secondary structure of newly synthesized chains.

Authors:  R Okazaki; T Okazaki; K Sakabe; K Sugimoto; A Sugino
Journal:  Proc Natl Acad Sci U S A       Date:  1968-02       Impact factor: 11.205

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  An efficient mRNA-dependent translation system from reticulocyte lysates.

Authors:  H R Pelham; R J Jackson
Journal:  Eur J Biochem       Date:  1976-08-01

6.  [Demonstration of RNA synthesis during segmentation in the Axolotl embryo].

Authors:  J Signoret; J Lefresne
Journal:  C R Acad Hebd Seances Acad Sci D       Date:  1975-07-28

7.  Specificity of oligo (dT)-cellulose chromatography in the isolation of polyadenylated RNA.

Authors:  J A Bantle; I H Maxwell; W E Hahn
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

Review 8.  DNA ligases of eukaryotes.

Authors:  S Söderhäll; T Lindahl
Journal:  FEBS Lett       Date:  1976-08-01       Impact factor: 4.124

9.  Size distribution and stability of DNA-like RNA synthesized during development of anucleolate embryos of Xenopus laevis.

Authors:  D D Brown; J B Gurdon
Journal:  J Mol Biol       Date:  1966-08       Impact factor: 5.469

10.  Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.

Authors:  H Aviv; P Leder
Journal:  Proc Natl Acad Sci U S A       Date:  1972-06       Impact factor: 11.205
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  4 in total

Review 1.  Early transcription in different animal species: implication for transition from maternal to zygotic control in development.

Authors:  Yannick Andéol
Journal:  Rouxs Arch Dev Biol       Date:  1994-01

Review 2.  Early transcription in different animal species: implication for transition from maternal to zygotic control in development.

Authors:  Yannick Andéol
Journal:  Rouxs Arch Dev Biol       Date:  1994-10

3.  Expression of DNA ligase genes by ram spermatid nuclei and RNA in amphibian eggs.

Authors:  J C David; M Loir; J Lefresne; P Thiebaud; J Signoret
Journal:  Rouxs Arch Dev Biol       Date:  1986-04

4.  Reinvestigation of DNA ligase I in axolotl and Pleurodeles development.

Authors:  S Aoufouchi; S Hardy; C Prigent; M Philippe; P Thiebaud
Journal:  Nucleic Acids Res       Date:  1991-08-25       Impact factor: 16.971
  4 in total

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