Literature DB >> 6682712

A survey of readily available chelators for buffering calcium ion concentrations in physiological solutions.

A C Durham.   

Abstract

Stability constants are reported for the binding of H+, Ca2+ and Mg2+ ions to the chelators commonly abbreviated EGTA, EDTA, HEDTA, DPA, NTA, ADA, and citrate, under uniform conditions of physiological temperature and ionic strength. Other compounds usable as calcium buffers are listed. The theoretical and practical considerations that influence the actual pCa attained in a chelator solution are discussed and a Hepes-buffered saline solution is suggested as a standard of "physiological pH". With these figures it is possible to make a rational choice of chelator to control the pCa and pMg of solutions for investigations in cell physiology, drug action, virus reproduction, and ion binding to proteins.

Entities:  

Mesh：

Substances：

Year:  1983        PMID： 6682712     DOI： 10.1016/0143-4160(83)90047-7

Source DB:  PubMed          Journal:  Cell Calcium        ISSN： 0143-4160            Impact factor:   6.817


  22 in total

1.  Charybdotoxin blocks with high affinity the Ca-activated K+ channel of Hb A and Hb S red cells: individual differences in the number of channels.

Authors:  D Wolff; X Cecchi; A Spalvins; M Canessa
Journal:  J Membr Biol       Date:  1988-12       Impact factor: 1.843

2.  Citrate Anticoagulant Improves the Sensitivity of Borreliella (Borrelia) burgdorferi Plasma Culture.

Authors:  Adriana R Marques; Xiuli Yang; Alexis A Smith; Xuran Zhuang; Siu-Ping Turk; Carla D Williams; Melissa A Law; Alan G Barbour; Utpal Pal
Journal:  J Clin Microbiol       Date:  2017-09-06       Impact factor: 5.948

3.  Effects of D2O on permeation and gating in the Ca(2+)-activated potassium channel from Chara.

Authors:  I I Pottosin; P R Andjus; D Vucelić; G N Berestovsky
Journal:  J Membr Biol       Date:  1993-11       Impact factor: 1.843

4.  Effects of Ca2+ and Mg2+ on the activity of pyruvate dehydrogenase phosphate phosphatase within toluene-permeabilized mitochondria.

Authors:  P J Midgley; G A Rutter; A P Thomas; R M Denton
Journal:  Biochem J       Date:  1987-01-15       Impact factor: 3.857

5.  Human axons contain at least five types of voltage-dependent potassium channel.

Authors:  G Reid; A Scholz; H Bostock; W Vogel
Journal:  J Physiol       Date:  1999-08-01       Impact factor: 5.182

6.  Enzymic characterization of epidermis-derived 12-lipoxygenase isoenzymes.

Authors:  M Siebert; P Krieg; W D Lehmann; F Marks; G Fürstenberger
Journal:  Biochem J       Date:  2001-04-01       Impact factor: 3.857

7.  Divalent cation chelators citrate and EDTA unmask an intrinsic uncoupling pathway in isolated mitochondria.

Authors:  Anatoly A Starkov; Christos Chinopoulos; Natalia N Starkova; Csaba Konrad; Gergely Kiss; Anna Stepanova; Vasily N Popov
Journal:  J Bioenerg Biomembr       Date:  2016-03-14       Impact factor: 2.945

8.  Purification of a 100 kDa phospholipase A2 from spleen, lung and kidney: antiserum raised to pig spleen phospholipase A2 recognizes a similar form in bovine lung, kidney and platelets, and immunoprecipitates phospholipase A2 activity.

Authors:  D K Kim; J V Bonventre
Journal:  Biochem J       Date:  1993-08-15       Impact factor: 3.857

9.  A procedure for the rapid isolation from rat liver of plasma membrane vesicles exhibiting Ca2+-transport and Ca2+-ATPase activities.

Authors:  R J Epping; F L Bygrave
Journal:  Biochem J       Date:  1984-11-01       Impact factor: 3.857

10.  Abnormality of phospholipid transverse diffusion in sickle erythrocytes.

Authors:  A Zachowski; C T Craescu; F Galacteros; P F Devaux
Journal:  J Clin Invest       Date:  1985-05       Impact factor: 14.808
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.