Literature DB >> 6671366

Improved immunologic detection of Clostridium difficile antigen by counterimmunoelectrophoresis.

R W Ryan, I Kwasnik, R C Tilton.   

Abstract

An improved counterimmunoelectrophoresis (CIE) procedure for detection of Clostridium difficile antigen is described. Commercially available antiserum to C. difficile toxin was absorbed with whole cells of C. difficile. CIE (absorbed) was 100% sensitive and 77.5% specific when compared to the tissue culture toxin assay. Instances are noted in which the CIE (absorbed) and/or bacterial culture was positive and the tissue culture assay was negative.

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Year:  1983        PMID： 6671366     DOI： 10.1016/0732-8893(83)90033-0

Source DB:  PubMed          Journal:  Diagn Microbiol Infect Dis        ISSN： 0732-8893            Impact factor:   2.803


  4 in total

Review 1.  Clostridium difficile: clinical disease and diagnosis.

Authors:  F C Knoop; M Owens; I C Crocker
Journal:  Clin Microbiol Rev       Date:  1993-07       Impact factor: 26.132

2.  Detection of Clostridium difficile toxins by enzyme immunoassay.

Authors:  C Krishnan
Journal:  J Hyg (Lond)       Date:  1986-02

3.  Latex particle agglutination for detecting and identifying Clostridium difficile.

Authors:  R A Bowman; S A Arrow; T V Riley
Journal:  J Clin Pathol       Date:  1986-02       Impact factor: 3.411

4.  Latex agglutination test for detection of Clostridium difficile toxin in stool samples.

Authors:  M S Shahrabadi; L E Bryan; D Gaffney; S E Coderre; R Gordon; C H Pai
Journal:  J Clin Microbiol       Date:  1984-09       Impact factor: 5.948
  4 in total

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