A simplified technique for fertilization and culture of human preimplantation embryos in vitro.

The paper describes simplification of existing methods of culture in human IVF. Focusing attention on the need to streamline procedures, the mode of housing the egg/embryo in a constant accessible environment is examined. We now culture with B2/B3-Menezo medium in a multidish under 5% CO2 in air at 37 degrees C in an open system within a CO2 gassing incubator. The advent of the multidish as a specific housing system is highlighted. The 80% fertilization rate combined with a stable osmolarity demonstrates that previous success is maintained with changes introduced to improve convenience and simplicity of procedures.