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Literature DB >> 6668476

Long-term culture of human oligodendrocytes. Isolation, growth and identification.

S U Kim, Y Sato, D H Silberberg, D E Pleasure, L B Rorke.

Abstract

Oligodendrocytes were isolated from adult human brains obtained at autopsy. The cells were prepared by Percoll density gradient centrifugation, seeded on plastic coverslips and were cultured for a period up to 6 months. The oligodendrocytes in culture expressed cell-type specific markers, galactocerebroside and myelin basic protein and revealed the ultrastructure of mature oligodendrocytes.

Entities: Chemical Gene Species

Mesh：

Year: 1983 PMID： 6668476 DOI： 10.1016/0022-510x(83)90206-x

Source DB: PubMed Journal: J Neurol Sci ISSN： 0022-510X Impact factor: 3.181

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Cited

13 in total

1. Cytotropism of Theiler's murine encephalomyelitis viruses in oligodendrocyte-enriched cultures.

Authors: Y Ohara; H Konno; Y Iwasaki; T Yamamoto; H Terunuma; H Suzuki
Journal: Arch Virol Date: 1990 Impact factor: 2.574

2. Double-stranded RNA unwinding and modifying activity is detected ubiquitously in primary tissues and cell lines.

Authors: R W Wagner; C Yoo; L Wrabetz; J Kamholz; J Buchhalter; N F Hassan; K Khalili; S U Kim; B Perussia; F A McMorris
Journal: Mol Cell Biol Date: 1990-10 Impact factor: 4.272

3. Long-term cultures of human adult Schwann cells isolated from autopsy materials.

Authors: G Moretto; S U Kim; D H Shin; D E Pleasure; N Rizzuro
Journal: Acta Neuropathol Date: 1984 Impact factor: 17.088

4. Gradient isolation of glial cells: evidence that flat epithelial cells are astroglial cell precursors.

Authors: K Meller
Journal: Cell Tissue Res Date: 1987-07 Impact factor: 5.249

5. Myelin basic protein does not have a mitogenic effect on adult oligodendrocytes.

Authors: G R Moore; S U Kim; E Chang; M Kim
Journal: Acta Neuropathol Date: 1995 Impact factor: 17.088

6. Human microglial cell isolation from adult autopsy brain: brain pH, regional variation, and infection with human immunodeficiency virus type 1.

Authors: Kimberly Schuenke; Benjamin B Gelman
Journal: J Neurovirol Date: 2003-06 Impact factor: 2.643

Long-term culture of human oligodendrocytes. Isolation, growth and identification.

1. Cytotropism of Theiler's murine encephalomyelitis viruses in oligodendrocyte-enriched cultures.

2. Double-stranded RNA unwinding and modifying activity is detected ubiquitously in primary tissues and cell lines.

3. Long-term cultures of human adult Schwann cells isolated from autopsy materials.

4. Gradient isolation of glial cells: evidence that flat epithelial cells are astroglial cell precursors.

5. Myelin basic protein does not have a mitogenic effect on adult oligodendrocytes.

6. Human microglial cell isolation from adult autopsy brain: brain pH, regional variation, and infection with human immunodeficiency virus type 1.

7. Human oligodendrocytes in dissociated cell culture.

8. Culture and cryopreservation of adult human oligodendrocytes and astrocytes.

9. Inhibition of myelin formation by HIV-1 gp120 in rat cerebral cortex culture.

10. Inducibility of Ia antigen on astrocytes by murine coronavirus JHM is rat strain dependent.