| Literature DB >> 6668309 |
E A De Bruijn, O Driessen, N van den Bosch, E van Strijen, P H Slee, A T van Oosterom, U R Tjaden.
Abstract
A gas chromatographic assay for the determination of 5-fluorouracil (5-FU) and 5,6-dihydrofluorouracil (FDHU) is described. The selectivity and sensitivity of the method allows the determination of both 5-FU and FDHU in 200 microliters of plasma. Diphenylsuccinimide and chlorouracil were used as external and internal standard, respectively. The assay including the extraction shows a good linearity in the range 0-5000 ng/ml plasma for 5-FU as well as for FDHU. 5-FU and FDHU plasma concentrations of a number of patients with breast cancer treated with 5-FU were determined in order to demonstrate the usefulness of the method.Entities:
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Year: 1983 PMID： 6668309 DOI： 10.1016/s0378-4347(00)84787-8
Source DB: PubMed Journal: J Chromatogr