| Literature DB >> 6667508 |
H U Koop, J Dirk, D Wolff, H G Schweiger.
Abstract
Two single mesophyll-protoplasts of Nicotiana tabacum cv. xanthi were selected into a 100 nl microdroplet of 0.4 M mannitol. Two cylindrical platinum electrodes were inserted into the microdroplet to align the two single cells via dielectrophoresis in an AC-field (1 MHz, about 120 V X cm-1). A single square DC-pulse of about 1.5 KV X cm-1 was applied to induce protoplast fusion.Entities:
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Year: 1983 PMID： 6667508 DOI： 10.1016/0309-1651(83)90019-x
Source DB: PubMed Journal: Cell Biol Int Rep ISSN： 0309-1651